体外诱导骨髓基质细胞向心肌细胞分化的实验研究  

Experimental Study on Differentiation of Meseachymal Stem Cells Into Cardiomyocytes in Vitro

作　　者：孟自力[1] 沈振亚[1] 焦鹏[1] 李冰雁[2] 张增利[2] 于曙东[1] 余云笙[1] 叶文学[1] 藤小梅[1] 李红卫[1] 张卫民[1] 刘鸿程[1] 黄浩岳[1] 

机构地区：[1]苏州大学附属第一医院心血管外科,江苏苏州215006 [2]苏州大学核医学院,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2004年第4期499-501,共3页Suzhou University Journal of Medical Science

摘　　要：目的　探讨体外诱导骨髓基质细胞向心肌细胞转化的条件。方法　分离大鼠骨髓 ,体外培养、传代得到骨髓基质细胞 ,观察不同浓度的诱导剂 5 氮胞苷对骨髓基质细胞的生长和诱导作用 ,进行形态学和免疫组织化学鉴定。结果　 5 氮胞苷诱导骨髓基质细胞转化为心肌样细胞 ,在诱导后 2周转化率为 2 9%。心肌特异性肌钙蛋白T(TnT)免疫组织化学染色阳性。结论　MSCs在体外条件下经 5 氮胞苷诱导可分化成心肌样细胞。Objective To study the differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes by 5-azacytidine (5-aza) in vitro. Methods Rat MSCs were isolated and cultured,then co-cultured with 5-aza at different concentration for 24 h. After cultured for additional 2 weeks,MSCs were identified by means of immunochemistry technique. Results A part of the cells affected by 5-aza had morphological changes and 29% of it was stained positively for Tn T. Conclusion MSCs can be induced into cardiomyocytes by 5-aza in vitro.

关 键 词：骨髓基质细胞 心肌样细胞 分化/细胞 细胞培养 5-氮胞苷 

分 类 号：R654.2[医药卫生—外科学]