6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达  

Cloning,sequencing and expression of glucitol-6-phosphate dehydrogenase(gutD) gene

作　　者：陈启伟[1] 刘广发[1] 邱凤英[2] 

机构地区：[1]厦门大学生命科学学院微藻工程研究所,福建厦门361005 [2]福建农林大学,福建福州350002

出　　处：《台湾海峡》2004年第3期314-317,共4页Journal of Oceanography In Taiwan Strait

摘　　要：本研究根据6 磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物,以假单胞杆菌(PseudonmonasXM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析.将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Es cherichiacoli)JM101后检测其蛋白表达及菌株生长耐盐性.A glucitol-6-phosphate dehydrogenase (gutD) was cloned viaPCR method using Pseudomonas genomic DNA as template. The gutD gene was sequenced and expressed in pBV220 vector. The transformant was tested for its salty tolerance.

关 键 词：分子生物学 6-磷酸山梨醇脱氢酶基因 克隆 序列分析 蛋白表达 

分 类 号：Q7[生物学—分子生物学]