NDV HeB02分离株的生物学特性及其F基因的克隆与序列测定  

The Biological Characteritics of NDV Heb02 Isolate and Its Gene Cloning and Sequence Analysis

作　　者：孙一敏[1] 于秀俊[1] 柏佳宁[1] 孙继国[2] 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]河北农业大学动物科技学院,保定071000

出　　处：《微生物学通报》2004年第4期5-8,共4页Microbiology China

基　　金：河北师范大学分子细胞生物学重点学科基金资助项目 (No 2 0 0 1 0 2 )

摘　　要：对河北省新城疫分离株HeB0 2的生物学特性进行了鉴定 ,根据国外已发表的新城疫病毒F基因序列 ,设计了一对引物并以RT PCR特异性扩增出HeB0 2分离株的F基因 ,基因产物大小为 1 63kb ,与设计相符 ,对其进行序列测定后 ,与其它标准毒株F48E9、LaSota和Clone30的F基因进行同源性比较 ,结果表明 ,HeB0 2株与国内标准强毒株F48E9及目前广泛应用的弱毒疫苗LaSota和Clone30的F基因核苷酸序列的同源性分别为 88 1 %、84 9%和 83 8%,由此可以看出HeB0 2分离株与标准毒株和疫苗株在F基因上发生了变异。The biological characteristics of NDV HeB02 isolate were identified One pair of primers for amplifying the F gene of NDV had been designed, with which the predicted 1 63 kb fragment had been amplified via RT PCR from NDV strain HeB02 The F gene of strain HeB02 was sequenced and compared with standard strains such as F48 E9, La Sota and Clone30 The results showed that the homologies of nucleic acids between HeB02 strain and F48 E9 , La Sota and Clone30 strains were 88 1 %?84 9 % and 83 8 % respectively The difference of the F gene of NDV strain HeB02 with those of other NDV standard strains had been proved

关 键 词：新城疫 生物学特性 F基因 序列分析 

分 类 号：S852.61[农业科学—基础兽医学]