白曲霉工程菌TR12循环转化酒精废液研究  

Study on the Condition of Cyclic Transformation of Ethanol Distilled Wastewater by Genetic Engineering Strain TR12

作　　者：向文良[1] 张文学[1] 乔宗伟[1] 胡承[1] 

机构地区：[1]四川大学轻纺与食品学院,成都610065

出　　处：《微生物学通报》2004年第4期26-29,共4页Microbiology China

基　　金：教育部留学回国人员启动基金 (No 2 0 0 0 3 67)

摘　　要：在含固体残渣 8%的酒精废液中 ,加入尿素 2g/L、玉米浆 2mL/L ,经接种白曲霉基因工程菌TR1 2菌株 ,在 32℃摇床培养 70h后 ,可获得含糖化酶 2 4 6U/mL和耐酸性α 淀粉酶 1 3 42U/mL的转化液。转化液直接循环利用于无蒸煮酒精发酵配料工序 ,不仅不影响酒精生产的产量和质量 ,而且能有效地减轻环境污染压力 ,节约水资源 。Five grams of urea and two milliliters of corn syrup were added into 1 liter ethanoldistilled wastewater with 8% solid dregs The activity of acid resistant α amylase and glucoamylase produced by inoculated Aspergillus kawachii genetic engineering strain TR12 in the transformed liquid reached a level of 13 42U/mL and 246U/mL respectively after fermentation at 32℃ by the shaking flask for 70 hours The transformed liquid was circularly applied to the ethanol ingredient process without cooking room, not only it didn't influence the ethanol output and quality, but also it could efficiently reduce the pollution of ethanol distilled wastewater and the cost of ethanol production

关 键 词：酒精废液 微生物转化 转化液回用 

分 类 号：X703[环境科学与工程—环境工程]