担子菌草菇总RNA的快速抽提方法  

Rapid Isolation of Total RNA from Basidomycetes Volvariella volvacea

作　　者：李迅[1] 裴建军[2] 邵蔚蓝[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214036 [2]南京师范大学生命科学学院,南京210097

出　　处：《微生物学通报》2004年第4期81-84,共4页Microbiology China

基　　金：国家自然科学基金资助项目 (NO . 3 0 3 70 0 3 4)~~

摘　　要：针对高等真菌草菇 (Volvariellavolvacea) ,提供一种改进的简易抽提总RNA的方法。纯化的RNA可直接作如下的各种应用 ,如RT PCR、polyA RNA的富集、差异显示、North ern杂交、引物延伸、cDNA合成、基因表达谱芯片和基因表达谱芯片分析。我们用此方法成功地从草菇中抽提到总RNA ,A2 60 /A2 80 为 1 7～ 2 0 ,A2 60 /A2 30 大于 2 0 ,数值显示RNA的纯度是足够高的 (RNA没有被蛋白或苯酚等污染 )。电泳图上 2 8S∶1 8S比例约为 2∶1 ,显示RNA没有被降解。We provide a modified and simple method to isolate the total RNA from basidiomycetes Volvariella volvacea The purified RNA is ready for use in standard and downstream applications such as RT PCR, poly A RNA selection, differential display, Northern dot and slot blotting, primer extension, cDNA synthesis, expression array and expression chip analysis We have successfully isolated total RNA from Volvariella volvacea with the way for many times The value of A 260 / A 280 fell in the range from 1 7 to 2 0 and that of A 260 / A 230 is larger than 2 0 which indicated that the purity of RNA was satisfactorily high (RNA was not contaminated by proteins or benzene phenol etc ) Electrophoresis analyzing total RNA sample show total RNA hasn't been broken down because 28S∶18S is equal to 2∶1

关 键 词：担子菌 草菇 总RNA 

分 类 号：Q781[生物学—分子生物学]