小麦叶片蛋白质双向电泳的改良方法  

An Improving Method of Two-D Gel Electrophoresis for Analyzing the Protein in Wheat Lamina

作　　者：朱宏[1] 张中恒 王同昌[1] 

机构地区：[1]哈尔滨师范大学,哈尔滨150080 [2]克山师范专科学校

出　　处：《东北林业大学学报》2004年第4期68-69,共2页Journal of Northeast Forestry University

基　　金：黑龙江省自然科学基金资助项目;哈尔滨师范大学博士科研启动基金资助项目

摘　　要：以普通小麦叶片为实验材料 ,在原有双向电泳基础上通过对样品制备、样品溶解、等电聚焦电泳、SDS-聚丙烯酰胺凝胶电泳以及染色方法等关键步骤进行改进和优化 ,在小麦叶片可溶性蛋白的分析中获得了满意的双向电泳图谱。By using the lamina from Triticum aestivum as experimental material, a satisfied picture of two-D gel electrophoresis was obtained based on the analysis of its soluble protein through improving and optimizing the key procedures, such as sample preparation, sample dissolution, IEF electrophoresis, SDS-PAGE and dyeing routine. This research result will provide a foundation for primarily analyzing the protein in wheat and other plants.

关 键 词：双向电泳 等电聚焦电泳 SDS-聚丙烯酰胺凝胶电泳 

分 类 号：S132[农业科学—农业基础科学]