烟草和酒精联合应用对遗传物质损伤的研究  

Effect of Combination of Tobacco and Alcohol on Genetic Material

作　　者：贲亚利[1] 朱达利[2] 董长垣[1] 周风生[2] 游艾青[3] 刘军[1] 

机构地区：[1]武汉大学医学院病毒学研究所病毒与癌研究室,武汉430071 [2]江汉大学,武汉430016 [3]湖北省农业科学院,武汉430000

出　　处：《武汉大学学报（医学版）》2004年第5期511-513,共3页Medical Journal of Wuhan University

摘　　要：目的 :研究烟草和酒精对遗传物质损伤是否存在联合效应。方法 :采用SCE、微核、Ames试验和CHO细胞集落形成试验等方法 ,对实验结果进行单因素方差分析和q检验。结果 :发现酒精和烟草都有显著提高SCE频率和微核形成率的作用 ;烟草能显著地提高伤寒沙门氏菌突变率和CHO细胞集落形成率 ;酒精烟草联合各指标均高于酒精或烟草。结论 :酒精与烟草对遗传物质损伤存在叠加效应。Objective: To study whether tobacco and alcohol can cause the combined effect to the genetic material. Methods: The frequency of sister chromatid exchange(SCE), micronucleus(MN), the Ames test and CHO colony forming test were used,and these results were analyzed by one-way ANOVA and q-test. Results: It was found that either tobacco or alcohol could increase the frequency of SCE and MN formation. Tobacco increased significantly the mutation rate of Salmonella typhia and the formation rate of CHO colony. Tobacco combined with alcohol could increase these four indexes to higher degrees than tobacco or alcohol solely did.Conclusion: Those results show that tobacco and alcohol have the combined effect to induce the injury of genetic materials.

关 键 词：酒精 烟草 SCE 微核 AMES试验 集落试验 

分 类 号：R373[医药卫生—病原生物学]