柱前衍生HPLC法测定鸦胆子油中的脂肪酸含量  

Determination of fatty acids in Fructus Bruceae oil by precolumn derivation HPLC

作　　者：丁怡[1] 唐星[1] 

机构地区：[1]沈阳药科大学药剂教研室,辽宁沈阳110016

出　　处：《中草药》2004年第9期988-991,共4页Chinese Traditional and Herbal Drugs

基　　金：辽宁省重大课题项目(2002226005)

摘　　要：目的建立HPLC法测定鸦胆子油中的脂肪酸的方法。方法以2,4′-二溴苯乙酮为衍生化试剂,18-冠-6醚为相转移催化剂,采用KromasilC8(250mm×4mm,5μm)反相柱,波长254nm,以乙腈-水(80∶20)为流动相等度洗脱,柱温室温,体积流量为1.3mL/min,正十七烷酸为内标,一次基线分离5种脂肪酸。结果亚油酸的线性范围为0.022～0.330μg,软脂酸的线性范围为0.014～0.213μg,油酸线性范围为0.028～0.416μg,硬脂酸的线性范围为0.012～0.177μg。平均回收率分别为99.2%、97.2%、101.8%、97.8%,RSD分别为1.2%、1.5%、0.4%、2.3%。结论该方法重现性好,定量准确,可作为鸦胆子油中脂肪酸的定量方法。Object A method was developed for the determination of fatty acid in Fructus Bruceae oil by precolumn derivation HPLC Methods Fatty acids were derivatized with p bromophenacylbromide as derivative and 18 crown 6 as catalyst The method used C 8 Kromasil C 8 (250 mm×4 mm, 5 μm) column and isocratic acetonitrile water eluent and the internal standard was heptadecanoic acid The detection wavelenghth was 254 nm Column temperature was fixed at room temperature, and the flow rate was 1 3 mL/min Results The standard curves of linoleic, palmitic, oleic, and steraric acid are linear within the range of 0 022—0 330, 0 014—0 213, 0 028—0 416, and 0 012—0 177 μg, respectively The four fatty acid recoveries are 99 2%, 97 2%, 101 8%, 97 8%, and the RSD are 1 2%, 1 5%, 0 4%, 2 3%, individuslly Conclusion Five fatty acids are separated within 30 minutes during a single run The present method is reliable and relatively simple for the determination of fatty acid in Fruetus Bruceae oil

关 键 词：鸦胆子油 脂肪酸 柱前衍生化 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]