发育中神经元惊厥性损伤的相关基因表达  

Expressions of injury-related gene in cultured developing neurons following seizures

作　　者：曹海燕[1] 王静敏[1] 姜玉武[1] 潘虹[1] 薄涛[1] 吴希如[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《实用儿科临床杂志》2004年第9期765-767,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金资助项目 (30 2 70 4 4 8)

摘　　要：目的　研究惊厥发生后不同发育阶段神经元白细胞介素 1受体 (IL 1R)、Cx36基因表达。方法　无镁细胞外液短暂处理培养的发育中皮层神经元 ,用Real time定量RT PCR方法检测IL 1R、Cx36mRNA表达改变。结果　 1.体外培养 6、17d神经元无镁处理后IL 1R表达呈一过性降低 ,之后 6d神经元表达恢复正常 ,而17d神经元表达增加 ,于处理后 2 4h达高峰 (P <0 .0 5 )。 2 .体外 6d的神经元无镁处理后Cx36表达渐增高 ,于处理后 2 4h达高峰。体外 17d神经元处理后 6hCx36表达与对照组比较明显降低 (P <0 .0 5 ) ,2 4h降低达峰值。结论　无镁诱导惊厥后体外 6、17d神经元IL 1R、Cx36表达不同 ,可能与惊厥造成 6。Objective To study interleukin-1 receptor(IL-1R) and connexin(Cx36) gene expression following Mg 2+-free-induced seizures in cultured developing neuron. Methods Rat embryo cortical neurons cultured for 6 and 17 days were exposed to Mg 2+-free media to induce seizure. At different time after Mg 2+-free treatment, real-time RT-PCR was used to detect IL-1R and Cx36 mRNA expression. Results 1. IL-1R mRNA expression transiently decreased after Mg 2+-free treatment in neurons cultured for 6 and 17 days in vitro. Then the levels of IL-1R mRNA expression recovered in neurons cultured for 6 days, but IL-1R mRNA expression were increased in neurons cultured for 17 days compared with control group and the peak was at 24 hours. 2. In neurons cultured for 6 days in vitro, Cx36 mRNA expression increased after Mg 2+-free treatment compared with control group, the peak was at 24 hours. But in neurons cultured for 17 days in vitro, Cx36 mRNA expression decreased at 6 hours after Mg 2+-free treatment compared with control group, the peak was at 24 hours. Conclusions IL-1R mRNA and Cx36 mRNA expression following Mg 2+-free-induced seizures are different between the neurons cultured for 6 and 17 days in vitro. This is possibly related to the different neuron injury between 6 and 17 days in vitro following seizures.

关 键 词：惊厥 发育 白细胞介素1受体 缝隙连接 基因 

分 类 号：R720.597[医药卫生—急诊医学]