褪黑素单克隆抗体的制备及鉴定  

The production and characterization of monoclonal antibodies to MT

作　　者：王建和[1] 袁均林[2] 杨唐斌[3] 曲丽娜[3] 顾洪雁[2] 陈正望[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074 [2]华中师范大学生命科学学院,武汉430079 [3]中国航天医学工程研究所,北京100960

出　　处：《华中师范大学学报（自然科学版）》2004年第3期355-357,共3页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(200372023).

摘　　要：为了获得抗褪黑素(MT)高效价的单克隆抗体,用甲醛将MT连结到BSA上,然后用连结物背部皮下多点免疫Balb/c小鼠,用PEG诱导免疫鼠脾细胞与Sp2/0细胞融合,经ELISA法筛选阳性克隆株及测定效价,用抗体与同类物之间的交叉反应进行特异性鉴定.最后获得5株(4C9D7、3E12C7H11E7、3E12A9D7、6A6A3A2C3、1E1E2H2H6)能稳定分泌高效价抗MT的单克隆抗体(McAb)的杂交瘤细胞.In order to obtain high titer monoclonal antibodies to MT, coupling MT to bovine serum albumin with formaldehyde and by immunizing Balb/c mice with multifocal intra-dermal injections with this MT conjugate., by Peg-induced fusion of immune PLN and Sp2/0 cells, we selected the positive cell strains and determined the titer of the McAb to MT. We evaluated the specificity of antibodies by determination of cross-reactivity of several analogues, we obtained 5 strains of hybridoma (4C9D7,3E12C7H11E7,3E12A9D7,6A6A3A2C3,1E1E2H2H6) whitch stably secreted higher titer monoclonal antibodies to melatonin.

关 键 词：褪黑素 单克隆抗体 免疫 小鼠 

分 类 号：Q57[生物学—生物化学]