三七复合有效成分对恒河猴细菌感染性子宫出血模型内膜组织NF-κB活性的影响  

Influence of two kinds of active components from Panax notoginseng on NF-κB activity in inflammatory Metrorrhagia model of Macaca mulatta

作　　者：马红霞[1] 尤昭玲[2] 王若光[2] 谭朝阳[2] 段祖珍[2] 余建[2] 

机构地区：[1]广州中医药大学,广东广州510405 [2]湖南中医学院中西医结合临床教研室,湖南长沙410007

出　　处：《中医药学刊》2004年第9期1619-1621,共3页Study Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(3 0 2 7163 3 );湖南省社会发展重大项目 (0 1SSY10 0 2 -4 )

摘　　要：目的 :探讨三七复合有效成分对恒河猴细菌感染性子宫出血NF -κB活性的影响。方法 :选择月经正常雌性育龄恒河猴 11只 ,随机分为中药组 (三七复合有效成分 ) 4只、模型组 4只及正常对照组 3只。用电泳迁移率改变分析法 (EMSA)检测三七复合有效成分组、模型组及正常对照组子宫内膜组织NF -κB与靶基因DNA的结合活性。结果 :三七复合有效成分组NF -κB活性明显低于模型组 ,三七复合有效成分组与正常对照组无显著性差别。结论 :NF -κB活化可能参与恒河猴细菌感染性子宫出血发病机制 ,三七复合有效成分对恒河猴细菌感染性子宫出血的止血作用可能与其抑制NFObjective:To explore influence of two kinds of active components from Panax notoginseng on NF-κB activity in inflammatory Metrorrhagia model of Macaca mulatta.Method:Eleven normal menorrhea adult Rhesus monkeys are choosed as research object,which are divided into four model groups,four TCM group and three Control guoup at random.Electrophoretic mobility shift assay(EMSA) was performed on the nuclear extracts to detect the activity of NF-κB in TCM group?model group and normal group.Result:compared to model group,the NF-κB activity of TCM group was weaker,the NF-κB activity of TCM group is similar to that of normal group.Conclusion:The activation of NF-κB is a key factor in infection-induced metrorrhagia.The reason two kinds of active components from Panax notoginseng decrease metrorrhagia is perhaps that it can inhibit the activation of NF-κB.

关 键 词：核因子-κB 子宫出血 三七 恒河猴 

分 类 号：R554[医药卫生—血液循环系统疾病]