重组冠状病毒核衣壳蛋白血清抗体检测  

Serum antibodies detection for nucleocapsid protein of SARS

作　　者：蒲丹 闫歌[1] 张秉强[1] 宋文鑫[1] 高小玲[1] 唐霓[1] 王丕龙[1] 郑建[1] 黄爱龙[1] 闫惠平[2] 檀玉芬[2] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,重庆400010 [2]首都医科大学附属北京佑安医院,北京100054

出　　处：《免疫学杂志》2004年第5期397-399,共3页Immunological Journal

基　　金：国家"8 63"高科技发展计划项目 (2 0 0 2AA2 1 50 1 5);重庆市科委项目 (7673)资助

摘　　要：目的　SARS冠状病毒的核衣壳 (Nucleocapsid)蛋白 (N蛋白 )是病毒的主要结构抗原 ,重组N蛋白可用作抗原检测患者血清中相应抗体。方法　以纯化的目的蛋白N 1,N 2分别包被 96孔板 ,检测正常人群及患者血清中抗SARS病毒抗体。结果　检测SARS感染患者血清 ,N 1阳性检出率为 5 5 .6 8% ,N 2阳性检出率为 5 6 .82 % ,与华大基因试剂盒相比符合率分别为 90 .12 %和 87.6 5 %。结论　重组核衣壳蛋白可做为检测SARS抗体的抗原蛋白。Objective To evaluate the specific antibody in the serum of SARS infected patients using recombinant nucleocapsid protein. Methods The 96-well plates were coated with recombinant N-1 and N-2 proteins, respectively. The specific serum antibodies in normal controls and SARS infected patients were assayed by ELISA, respectively. Results The positive rates of N-1 protein and N-2 protein in SARS infected patients were 55.68% and 56.82%, respectively. The concordance rates of N-1 and N-2 with the Huada kits were 90.12% and 87.65%, respectively. Conclusion The recombinant nucleocapsid protein could be used as an antigen for SARS diagnosis.

关 键 词：SARS 核衣壳蛋白 ELISA 抗体 

分 类 号：R446.61[医药卫生—诊断学]