免疫-PCR早期诊断肾综合征出血热的研究  

Study on immuno-PCR technique for early diagnosis of hemorrhagic fever with renal syndrome

作　　者：牟玲[1] 史俊岩[2] 王南[1] 刘祥宁 王晓梅[1] 杨晓光[3] 罗恩杰[2] 

机构地区：[1]沈阳市传染病院,110006 [2]中国医科大学病原生物学教研室 [3]沈阳市第九人民医院

出　　处：《中国公共卫生》2004年第6期689-690,共2页Chinese Journal of Public Health

基　　金：沈阳市科委研究基金资助项目 (2 0 0 1 4 1 9- 0 1 )

摘　　要：目的　建立一种敏感、特异的肾综合征出血热 (HFRS)早期诊断方法。方法　应用免疫 -PCR方法检测不同病程、临床诊断为HFRS的患者血中抗HFRS -IgM抗体 ,同时与ELISA和IFAT二种方法进行比较。 结果　免疫 -PCR检测 96份HFRS患者血清中抗HFRS -IgM抗体的阳性率为 76 % ,对 5 1份发热期患者的阳性检出率为6 2 7% ,而ELISA法和IFAT法对同批患者的阳性检出率分别为 5 7 3%和 39 2 % ,及 4 9%和 31 4 %。 2 0份甲型肝炎阳性血清、30份正常人血清及 2 0份HFRS -IgM阴性并掺入类风湿因子阳性的血清 ,免疫 -PCR检测均为阴性。结论　免疫 -PCR检测抗HFRS -IgM抗体敏感性高 ,特异性强 。Objective To seek a sensitive and specific method for early diagnosis of Hemorrhagic Fever with Renal Syndrome(HFRS).Methods The 96 sera from patients with HFRS diagnosed by clinic were tested with immuno-PCR techonique for anti HFRS-IgM antibody and compared with ELISA and IFAT,and a control group (20 sera with HAV positive,30 sera of normal human and 20 sera of HFRS IgM negative mixed with the sera of rheumatoid factor).Results For the 96 sera,the positive rate with Immuno-PCR was 76% and among the 96 sera,51sera were from patients with febrile stag and the positive rate with the immuno-PCR was 62.7%.For the latter two methods,the positive rate were 57.3% and 39.2%,or 49% and 31.4% respectively?20 sera with HAV positive,30 sera of normal human and 20 sera of HFRS IgM negative mixed with the sera of rheumatoid factor were all shown negative.Conclusion The method of Immuno -PCR for detection of anti HFRS-IgM antibody was sensitive and specific,which may become a new method for early diagnosis of HFRS.

关 键 词：免疫-PCR 肾综合征出血热 早期诊断 

分 类 号：R373.32[医药卫生—病原生物学]