大鼠颈上神经节脑内移植存活实验研究  

VIABILITY OF SUPERIOR CERVICAL GANGLIA TRANSPLANTED IN THE BRAIN OF ADULT RATS

作　　者：胡梅香[1] 罗兆明[2] 刘灶英[1] 

机构地区：[1]广州医学院组织胚胎学教研室,广州510182 [2]广州医学院解剖学教研室,广州510182

出　　处：《广东解剖学通报》1993年第1期46-50,共5页

摘　　要：本实验用幼年、年青和成年SD大鼠颈上神经节(SCG)分别移植于48只经6—OH-DA损毁黑质的同种成年鼠尾状核,存活1—2个月后,用尼氏染色和甲醛—戊二醛诱发单胺类荧光法对移植物进行了观察。结果18例移植幼鼠SCG者,移植物的节神经元几乎全部丧失;17例移植年青的SCG者,移植物中见有存活神经元的有9例,占52.94%;13例移植成年鼠SCG者,移植物中有存活神经元的有8例,占61.54%。存活神经元数量较少,且多分布在移植物的周围区,其胞质尼氏染色较深,荧光显微镜观察,显示指示CA存在的特异绿色荧光。在存活神经元存在的区域及该移植物与宿主脑组织之间的界面有大量毛细血管,并常有胶质细胞增生。本文对影响移植节神经元存活的因素进行了讨论。The superior cervical ganglia(SCG)of youngling,young adult and adult Sprague—Dawley rats were respectively homografted into the caudate nucleus of 48 adult rats in which the dopamine system in substantia nigra had been unilaterally destroyed by 6—hydroxydopamine.After survival period from 1 to 2 months,the grafted tissues treated by Nissl stain and formaldehyde,glutaraldehyde—induced monoamine fluorescencemethods were observed.Results showed that the ganglionic neurons of the youngling SCG transplanted in 18 recipients were almost all lost.Young adult SCG grafts in 9 of the 17 recipients showed surviving neurons(52.94%).The surviving neurons of adult SCG grafts in 8 of the 13 recipients could be observed(61.54%).The last two groups were not markedly dif- ferent(P>0.05).The surviving neurons were small in number.The surviving neurons were often distributed in the peripheral area of the grafts.Their cytoplasm was deeply stained by cresyl violet. Sometimes,the SIF cell might be seen.The areas that neurons had survived and the interface be- tween the host and graft had a large number of proliferating capillaries and glial cells.Under fluores- cence microscope,a even specific green fluorescence indicating the presence of catecholamine(CA)was observed in the surviving ganglionic neurons and SIF cells.This paper discussed the factors influencing the survive of transplanted ganglionic neurons.

关 键 词：颈 神经节 脑 移植术 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]