λDNA导入中国春小麦引起形态学及细胞学变化  

Studies on Morphologic and Cytological Changes of China Spring Wheat Introduced with λDNA

作　　者：高丽霞[1] 于元杰[1] 王波[2] 王希荣 

机构地区：[1]山东农业大学农学院,泰安271018 [2]山东农业大学机电学院,泰安271018 [3]山东东营市广北农场,东营257347

出　　处：《分子植物育种》2004年第5期637-642,共6页Molecular Plant Breeding

摘　　要：本研究将λDNA导入中国春小麦,对其后代D1,D2代的花粉母细胞(PMC)减数分裂行为进行了细胞学观察,并对形态性状的变异进行了跟踪统计.D1、D2代的减数分裂过程中均出现了单价体、染色体落后、染色体桥、多极分裂等异常现象.D1代PMC减数分裂变异率为25.54%,但未发现明显的形态性状变异.D2代PMC减数分裂变异率为24.05%,与D1代相比,D2代的PMC减数分裂变异开始分化,观察的出现PMC减数分裂变异的30个单株中变异幅度为9.04%～57.14%.D2代群体形态性状发生了丰富的变异,在株高、茎秆硬度、穗长、穗粒数等方面出现了变异,并且出现了茎秆坚硬、穗长增长、穗粒数增多的优良个体.In this study, we introduced λDNA into elite common wheat cultivar 'China spring', and observed the chromosome aberrance of D1 and D2 with λDNA introduced lines in the stage of pollen mother cell (PMC) meiosis. The results showed that both of the D1 and D2 lines had appeared the chromosome aberrances which including single chromosome, chromosome lagging, chromosome bridge, excessive bally dividing etc. The ratio of chromosome aberrance in D1 and D2 lines are 25.24% and 24.05%, respectively, and there are 9.04%~57.14% chromosome aberrance in observed 30 D2 individuals, which showed the differentiations of PMC in D2 lines comparing with D1 lines. In this research, we also identified the variations of agronomic traits of the D1 and D2 lines, it is obvious that there is few traits changes of D1 lines, but there are more changes of agronomic traits in D2 lines which including plant height, stem rigidity, panicle length, grain number per panicle etc. Some of agronomic traits changes could be used as breeding program such as plant height, harder stem, long panicle etc.

关 键 词：λDNA导入 中国 春小麦 形态学 细胞学 

分 类 号：S512.12[农业科学—作物学]