大豆抗孢囊线虫3号生理小种相关基因PCR检测方法的建立与应用  

Design and application of a PCR-based assay for resistant genes to race 3 of soybean cyst nematode in soybean

作　　者：陈观水[1] 周以飞[1] 潘大仁[1] 

机构地区：[1]福建农林大学作物科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2004年第3期359-362,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(B0210017).

摘　　要：根据甜菜的抗线虫病基因保守序列,设计合成了4对引物,从中筛选出1对引物,对8份经抗孢囊线虫3号生理小种常规鉴定的大豆品种(6份抗病、2份感病)进行了PCR检测.结果表明,6份抗病品种出现了1条600bp的特异片段,而2份感病品种未出现任何条带.这与常规鉴定结果相一致.PCR Sothern杂交验证了所获得特异片段与抗线虫病基因的同源性,证明使用该方法检测大豆抗孢囊线虫病基因简单、快速、方便、可行.Based on the sequence of cyst nematode resistant gene in sugar beet, 4 pairs of primers were designed. A pair of primers were selected among them. Eight soybean cultivars (including 6 nematode resistant varieties to SCN race 3, 2 susceptible varieties to SCN race 3 by conventional identification) were screened by using the pair of primers established for cyst nematode resistant gene in soybean. The result was in correspondence with that of conventional identification. PCR-Southern blot proved that it is a feasible, simple, rapid and applicable method for cyst nematode resistant gene in soybean.

关 键 词：大豆 孢囊线虫病 PCR检测 基因 品种 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] Q343.1[农业科学—植物保护]