日本血吸虫组织蛋白酶B DNA疫苗的免疫保护效果研究  

STUDY ON PROTECTIVE IMMUNITY OF CATHEPSIN B DNA VACCINE AGAINST SCHISTOSOMA JAPONICUM

作　　者：张冉[1] 易新元[2] 曾宪芳[2] 张顺科[2] Larry Mc Reynolds 

机构地区：[1]湖南师范大学医学院检验系 [2]中南大学湘雅医学院寄生虫学教研室 [3]Molecular Parasitology Unit,New England Biolabs,MA01915,USA

出　　处：《寄生虫与医学昆虫学报》2004年第3期139-143,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：WHO TDR项目 (No 980 2 5 5 );湖南省科委项目 (No 0 0jzy2 115 )资助

摘　　要：目的 :观察已构建日本血吸虫大陆株 31kDa组织蛋白酶BDNA疫苗VR1 0 1 2 Sj31及VR1 0 1 2 SjGST Sj31在BALB c小鼠体内的免疫保护效果。方法 :用纯化的空白质粒载体VR1 0 1 2、重组质粒VR1 0 1 2 Sj31及VR1 0 1 2 SjGST Sj31于股四头肌注射免疫BALB c鼠 :将 36只 6～ 8周龄BALB c鼠随机分为 3组 ,每组 1 2只 ,对照组 (A组 )注射空白质粒载体VR1 0 1 2 ,B组注射重组质粒VR1 0 1 2 Sj31 ,C组注射重组质粒VR1 0 1 2 SjGST Sj31。质粒剂量均为 1 0 0 μg ,每隔 2周免疫 1次 ,共免疫 3次 ,第 3次免疫后第 2 1天 ,每只鼠经腹部感染 1 0条尾蚴 ,4 2天后剖杀计数各小鼠成虫数和每克肝卵数 ,观察诱导产生的减虫和减卵效果 ,并用ELISA分析小鼠血清中的抗体。结果 :ELISA分析表明 ,第 3次免疫后小鼠出现特异性IgG抗体。与空白质粒对照组比较 ,2个实验组的减虫率分别为 2 5 0 %及 30 0 % (P <0 0 5 ) ,肝组织减卵率分别为 5 4 90 %及 6 9 74 % (P <0 0 0 1 ) ,每雌肝减卵率分别为 4 7 96 %、5 4 6 2 % (P <0 0 0 1 )。与B组相比 ,C组的肝组织减卵率为 32 92 % (P <0 0 0 1 ) ,每雌肝减卵率为 1 2 79% (P <0 0 5 )。结论 :DNA疫苗VR1 0 1 2 Sj31和VR1 0 1 2 SjGST Sj31能诱导小鼠产生一定水平的抗?Objective To observe the protective immunity induced by the DNA vaccines of VR1012 Sj31 and VR1012 SjGST Sj31 of Schistosoma japonicum (Sj)in BALB/c mice.Methods Thirty six BALB/c mice were divided into three groups.Of control group A,each mouse was injected at quadriceps femoris with 100μg VR1012 plasmid DNA;of group B,each mouse was injected intramuscularly with 100μg VR1012 Sj31 plasmid DNA;of group C,each mouse was injected intramuscularly with 100μg VR1012 SjGST Sj31 plasmid DNA.All mice were immunized three times with an interval of 2 weeks and were challenged each with 10 cercariae of Sj at the 21th days after final immunization.At day 42 after challenge,all mice were sacrificed,the numbers of worms and hepatic eggs were counted.Antibody level in the sera of mice before and three weeks after immunization was determined with ELISA.Results There are specific IgG in the sera of mice in the test groups after the 3rd immunization.Compared with the control group,the worm reduction were 25.0% and 30.0%( P <0.05);the egg reduction were 54.90% and 69.74%( P <0.001);the egg reduction rates in the liver per female worm were 47 96% and 54 62%( P <0 001)respectively.Compared with the B group,the egg reduction of C groups were 32 92%( P <0 001),the egg reduction rates in the liver per female worm were 12 79%( P <0 05).Conclusion The DNA vaccines,VR1012 Sj31 and VR1012 SjGST Sj31 could induce partial protective immunity against Sj in mice.The protective immunity induced by bi valent DNA vaccine was better than that induced by monovalent DNA vaccine.

关 键 词：日本血吸虫 组织蛋白酶B DNA疫苗 免疫 寄生虫病 

分 类 号：R392[医药卫生—免疫学]