成人骨髓间充质干细胞对脐血CD34^+细胞的体外扩增作用  

Ex vivo expansion of CD34^+ cells from human umbilical cord blood by adult bone marrow mesenchymal stem cells

作　　者：贾明峰[1] 侯相麟[1] 赵丽[2] 刘蓓[1] 李娟[2] 陈轩[2] 

机构地区：[1]兰州医学院第一附属医院血液科,甘肃兰州730000 [2]兰州医学院第一附属医院中心实验室,甘肃兰州730000

出　　处：《第四军医大学学报》2004年第18期1652-1655,共4页Journal of the Fourth Military Medical University

摘　　要：目的 :探讨骨髓间充质干细胞 (MSCS)对脐血CD34+细胞体外扩增作用 .方法 :分离培养成人MSCS 作为滋养层 ,联合SCF ,IL 11和GM CSF分别组成对脐血CD34+ 细胞的不同扩增体系 ,培养 3wk后观察脐血有核细胞、CFCs以及CD34+ 细胞扩增倍数的变化 .结果 :MSCS 联合细胞因子组较其他组培养体系对有核细胞总数、CFCs含量及CD34+ 细胞含量均具有明显的扩增作用 ,分别扩增了 114 .2± 2 .4 ,4 0 .5± 8.6 ,11.3± 0 .4倍 .结论 :成人MSCS 协同其他细胞因子可增强脐血CD34+ 细胞的体外扩增作用 .AIM: To investigate the effects of adult bone marrow mesenchymal stem cells on the expansion of umbilical cord blood CD34 + cells ex vivo. METHODS: Adult bone marrow mesenchymal stem cells were isolated and cultured as feeder layer. Umbilical cord blood CD34 + cells were cultured in combination with mesenchymal stem cells, stem cell factor, Interleukin-11 and granulocyte-macrophage colony stimulating factor, respectively. At the end of 3 weeks, the total number of nucleated cells , colony forming cells and CD34 + cells was repeatedly counted in culture system for 3 weeks to obtain the fold of expansion. RESULTS: The mesenchymal stem cells and cytokines co-culture systems significantly enhanced the expansion of the cord blood cells with the increase of the total number of nucleated cells by 114.2±2.4-fold, colony forming cells by 40.5±8.6-fold and CD34 + cells by 11.3±0.4-fold, respectively. CONCLUSION: The expansion of umbilical cord blood CD34 + cells can be enhanced ex vivo by adult bone marrow mesenchymal stem cells combined with cytokines.

关 键 词：体外扩增 CD34^+细胞 脐血 骨髓细胞 干细胞 

分 类 号：R331.2[医药卫生—人体生理学]