猪轮状病毒地方株JL94株VP4基因的克隆与序列分析  

Cloning of VP4 gene from porcine rotavirus JL94 isolate and homologous comparison with reference strains

作　　者：宋岩[1] 师东方[1] 李一经[1] 李慧昕[1] 李凯航[1] 秘晶纬[1] 

机构地区：[1]东北农业大学动物医学院病原分子生物学实验室,黑龙江哈尔滨150030

出　　处：《中国预防兽医学报》2004年第5期352-354,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省十五攻关课题 项目编号:GC01B510

摘　　要：根据GenBank已发表的猪轮状病毒VP4基因保守序列,利用Oligo4.1软件设计—对引物扩增猪轮状病毒地方株JL94株VP4全基因序列;并将扩增产物与pMD-18T载体连接,经鉴定后将重组质粒进行测序并与国外分离株CRW-8株、BEN-307株进行核苷酸和氨基酸序列同源性比较分析。结果表明:JL94株和CRW-8株、BEN-307株VP4全基因片段核苷酸同源性和氨基酸同源性分别为96.98％和98.05％,说明JL94株与CRW-8株、BEN-307株属于同一VP4血清型。本研究为进一步研制诊断性抗原和病毒重组活载体疫苗奠定了基础。A pair of primers according to the published cDNA sequence of porcine rotavirus VP4 gene were designed, which were used in cloning and sequence analysis of VP4 gene from porcine rotavirus JL94 isolated in China. The full length gene encoding outer capid protein VP4 was amplified from the native JL94 isolate of porcine rotavirus by reverse transcription polymerase chain reaction(RTPCR). The cDNA of VP4 gene was inserted into pMD-18T vector and identified by sequencing. The result of its homology comparison with CRW-8 strain and BEN-307 strain is 96.98 % and 98.05 % respectively.

关 键 词：猪轮状病毒 VP4基因 克隆 测序 

分 类 号：S852.65[农业科学—基础兽医学] Q785[农业科学—兽医学]