猪伪狂犬病高免血清的制作与初步应用  

The preparation and application of pig pseudorabies virus high immune serum

作　　者：孔令达[1] 符芳[1] 于辉[1] 崔尚金[1] 仇华吉[1] 韩英[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2004年第5期359-362,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：用伪狂犬病抗体阴性健康猪,以不同剂量、间隔一定时间、多次免疫接种伪狂犬病强毒S株,制备伪狂犬病病毒高免血清,试验表明,用本方法制备的伪狂犬病病毒高免血清特异性强、抗体效价高达256倍以上,完全符合诊断用血清标准要求。并将高免血清在中和试验、ELISA、免疫胶体金等进行了对比试验。This study produced the pseudorabies virus (PrV) high immune serum by multi-incubated the pseudorabies antibody negative healthy pig with strong pseudorabies vaccine strain S with different doses at certain intervals. And identified the high immune serum by neutralization test, ELISA and immuogold labeling test strip. The results approved that the PRV high immune serum produced by this method is of high specification, the titer is over 256 times, completely meet the needs of the diagnostic requirements.

关 键 词：伪狂犬病病毒 高免血清 ELISA 中和试验 免疫胶体金 

分 类 号：S852.65[农业科学—基础兽医学]