大黄提取物治疗犬肢体枪弹伤后脑神经元c-fos基因表达变化  

Alteration of c-fos gene expression in cerebral neuron with rhubarb extracts therapy following canine gunshot wounds in the limb

作　　者：封亚平[1] 章翔[2] 柏秀松[1] 费舟[2] 

机构地区：[1]成都军区昆明总医院神经外科,云南昆明650032 [2]第四军医大学西京医院全军神经外科研究所,陕西西安710032

出　　处：《创伤外科杂志》2004年第5期367-369,共3页Journal of Traumatic Surgery

基　　金：全军"九五"医学科研基金重点资助项目 (99Z14 6 )

摘　　要：目的　探讨中药大黄治疗犬肢体高能量枪弹伤后脑神经元c fos基因表达的变化。方法　 18只杂种犬 ,以M 193、5 .5 6mm制式弹射击犬双后肢肌肉丰满处 ,随机分为对照组、大黄治疗组。对照组伤后应用无菌注射用水鼻饲 ,大黄治疗组应用中药大黄的乙醇及水提液鼻饲。采用免疫组化 ,对两组伤后 2、6、10小时远隔部位脑组织脑神经元中c fos基因的表达分别进行检测。结果　大黄治疗组脑神经元中c fos基因表达较对照组显著减少 (P <0 .0 1)。结论　中药大黄能够抑制犬肢体枪弹伤后脑神经元c fos基因的表达。Objective To investigate the expression and change of regulation of c-fos gene on cerebral cortex after rhubarb extracts therapy following high-energy gunshot wounds in the extremity of dogs.Methods Eighteen dogs were divided into control groups and rhubarb therapeutic groups.M-193,5.56mm bullets were used to shoot the most plump part of the hind extremity of the dogs.The control groups received sterilized water by nasal feeding post-injury,alcohol and water solutions of rhubarb extracts by nasal feeding were administered to animals in the treatment group post-injury.C-fos genes expressions in neurons of cerebral tissues at 2,6 and 12h after gunshot in two groups were detected with immunohistochemistry.Results Expression of c-fos gene in the cerebral neurons in the rhubarb therapeutic groups were significantly lower than that found in the control group(P<0.01).Conclusion The Chinese medical herb,rhubarb extracts,can restrain c-fos gene expression in the neurons of cerebral tissues after the gunshot wounds in the limb.

关 键 词：火器伤 神经元 基因 大黄提取物 免疫组化 

分 类 号：R826.6[医药卫生—临床医学]