鸡副黏病毒YM毒株F基因的克隆及遗传进化关系研究  

The Choning and Sequence Analysis of F gene of Chicken paramyxovirus virulent YM Isolate

作　　者：陈全姣[1] 金梅林[1] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070

出　　处：《动物医学进展》2004年第5期63-67,共5页Progress In Veterinary Medicine

摘　　要：近年来,我国相继有新城疫强毒株出现的报道,为分析这些强毒株与疫苗株之间的遗传进化关系,研究新城疫的流行病学特点,文章以华中农业大学病毒室分离的YM毒株基因组RNA为模板,利用RT-PCR扩增F基因的cD-NA,将其连接入pMD18-T载体,经筛选证明已获得鸡副黏病毒YM毒株F基因的阳性克隆子。核苷酸序列和氨基酸序列分析表明,YM毒株属于NDV基因Ⅶ型强毒株;F蛋白氨基酸序列与中国标准强毒株F48E9 F蛋白氨基酸序列同源性为86％,与弱毒疫苗Lasota／46株氨基酸序列同源性仅为83％,证明为变异株,且其重要的抗原位点343位氨基酸由Leu→Pro,免疫原性发生了较大变化。从基因进化树也可见,其与中国标准强毒株F48E9,弱毒疫苗Lasota／46株的进化关系较远,这从分子角度解释了为什么高免的鸡仍然暴发新城疫。The F gene of virulent YM isolate of the chicken paramyxovirus was amplified by RT-PCR. The F gene fragment was cloned to pMD18-T vector. The result of sequence analysis indicated that the amino acids of cleavage site of F gene was 112RRQKF117, which match with the virulent NDV strains. Moreover, there are NDV geneⅦ characteristic K101 and V121 amino acid. Comparing other sequences in GenBank, it was found that there are 86 % and 83% amino acid sequence in homology with the virulent F48E9 isolate and avirulent La sota/46. The date showed that the YM isolate is a variation, using the DNASTAR, the analysis result suggested the immunity was changed, which explained in molecular why the chicken immunized infected the Newcastle.

关 键 词：鸡副黏病毒 YM毒株 F基因 基因克隆 遗传进化 序列分析 新城疫 

分 类 号：S858.31[农业科学—临床兽医学] S852.659.5[农业科学—兽医学]