两株诺瓦克样病毒新亚型的鉴定  

Identification of two new strains of norovirus in Guangzhou

作　　者：戴迎春[1] 聂军[1] 刘翼[1] 李志峰[1] 白杨 李建栋[1] 俞守义[1] 江熙 

机构地区：[1]第一军医大学流行病学教研室,广东广州510515 [2]辛辛那提儿科研究中心

出　　处：《第一军医大学学报》2004年第9期1016-1018,1022,共4页Journal of First Military Medical University

基　　金：广东省医学科研基金(A2003420)~~

摘　　要：目的对在广州检出的人类杯状病毒(HuCVs)毒株进行分子生物学鉴定,以确定其是否为新亚型。方法选择检出的HuCVs进行克隆、转化和序列分析,使用PHYLIP进行核酸序列同源性比较,经Treeview1.5绘制进化树。结果206份病毒性腹泻标本检出HuCVs18份,检出率为8.74%(18/206),经序列分析证实均为NVs GⅡ毒株。任选其中6株进行PCR产物的克隆和测序,与GenBank中的参考株比较并绘制遗传进化树,其中HuCV/NVGⅡ003/2003/CHN(32282)、HuCV/NVGⅡ004/2003/CHN(32283)与基因文库中病毒的最高同源性分别为83%和87%。结论广州存在其他国家或地区没有报道过的诺瓦克样病毒新亚型毒株。Objective To identify the molecular biological characteristics of human caliciviruses (HuCVs) and new strains of norovirus (NV) detected in Guangdong Province. Methods HuCVs were detected from the stool specimens by reverse transcription-PCR (RT-PCR), and the PCR products were cloned, transformed, and blasted with Genbank.The nucleotide sequence homology was also evaluated with PHYLIP package, and the phylogenetic tree was generated by Treeview. Results HuCVs were detected from 18 out of 206 stool specimens from children with viral diarrhea (8.74%), which were all confirmed subsequently as NVs. Two strains HuCV/NVGⅡ 003/2003/CHN(32282) and HuCV/NVGⅡ 004/2003/CHN(32283) from the selected 6 NVs strain were identified as new NV strains. Conclusion New NV strains in consistency with the blood-type distribution of Chinese are present in Guangzhou, which have not been reported in other countries.

关 键 词：人类杯状病毒 诺瓦克样病毒 基因亚型 腹泻 

分 类 号：R373[医药卫生—病原生物学]