DNA聚丙烯酰胺凝胶电泳银染方法的选择  

Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis

作　　者：李西平[1] 钱新华[1] 姚英民[1] 刘志杰[1] 刘阳[1] 

机构地区：[1]第一军医大学南方医院儿科,广东广州510515

出　　处：《第一军医大学学报》2004年第9期1072-1074,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(39770784)~~

摘　　要：目的比较4种聚丙烯酰胺凝胶银染方法,为初次使用者选择方法时提供参考。方法用24 mer的引物作为上样样本,分别采用氨水法、低浓度硝酸银法、0.1%及0.2%硝酸银法染色。后2种配合不同的显色剂显色。结果前2种未显色,0.1%及0.2%硝酸银可显色。结论0.2%的硝酸银与氢氧化钠显色剂相结合具有最高的灵敏度;0.2%的硝酸银与碳酸钠显色剂相结合具有最佳的显像效果。Objective To compare 4 silver staining methods for DNA detection in polyacrylamide gel electrophoresis. Methods After the electrophoresis was completed, the gels were stained separately by four different methods, namely using ammonia-silver-citric acid, low-concentration silver nitrate, 0.1% and 0.2% silver nitrate. Results DNA was detected only by staining with 0.1% and 0.2% silver nitrate. Conclusion 0.2% silver nitrate used along with sodium hydroxide shows the highest sensitivity in DNA detection, while its use with sodium carbonate produces the best quality of the image.

关 键 词：DNA 银染 聚丙烯酰胺凝胶电泳 

分 类 号：Q503[生物学—生物化学]