绵羊睾丸原代细胞培养及接种羊痘弱毒后的病变观察  

Culturing of ovine testicular cells and observation of pathological changes of the cells innoculated with attenuated sheep pox virus

作　　者：周建胜[1] 马慧玲[1] 郭庆山 

机构地区：[1]山东省畜牧兽医总站,山东济南250022 [2]山东省天厨集团技术部,山东济南250022

出　　处：《中国兽医科技》2004年第9期71-74,共4页Chinese Journal of Veterinary Science and Technology

摘　　要：研究了在相同培养条件下不同培养液、不同血清和不同细胞密度对绵羊睾丸细胞生长的影响 ,并对该细胞接种羊痘弱毒后的病变情况进行了观察。结果 ,3种培养液对绵羊睾丸细胞生长的支持作用表现为MEM液 >199液 >乳汉液 ,但差异不显著 ;用犊牛血清的最适细胞密度为8.0× 10 5～ 1.0× 10 6 /mL ,用成年牛血清的最适细胞密度为 1.0× 10 6 /mL ,用绵羊血清的最适细胞密度为 8.0× 10 5/mL ;Effect of cell culture media, sera and cell density on growth of ovine testicular cells was studied under the identical culturing condition and pathological changes of the cells inoculated with the (attenuated) sheep pox virus was observed. The results were as follows. The support effect of MEM, 199 and milk-Hank’s solutions on growth in vitro of the cells was obvious (in support order: MEM>199>milk-Hank’s (solution)). The most suitable densities of the cells in calf serum, mature bovine serum and (ovine) serum were 8.0×10~5-1.0×10~6/mL, 1.0×10~6/mL and 8.0×10~5/mL respectively. The grown-well ovine testicular cells appeared evident pathological changes after they were innoculated with the attenuated sheep pox virus.

关 键 词：绵羊睾丸细胞 细胞培养 细胞病变 

分 类 号：Q813.11[生物学—生物工程]