IL-12对SLE患者PBMC分泌IL-10、IFN-γ的影响  

Effect of Interleukin-12 on IL-10 and IFN-γ Production by PBMC in Patients Vitro

作　　者：张红娟[1] 王宗发[2] 彭学标[2] 杨健[1] 杨文林[1] 

机构地区：[1]广州医学院第二附属医院皮肤科,广东广州510260 [2]第一军医大学南方医院皮肤科,广东广州510515

出　　处：《中国皮肤性病学杂志》2004年第9期535-536,共2页The Chinese Journal of Dermatovenereology

摘　　要：目的　了解白细胞介素 12 (IL 12 )对SLE患者外周血单一核细胞 (PBMC)分泌白细胞介素 10 (IL 10 )、γ 干扰素 (IFN γ)的影响。方法　在培养体系中加入或不加入IL 12 ,观察IL 12对SLE患者及正常对照PBMC分泌IL 10 ,IFN γ的水平变化。结果　IL 12可使SLE患者PBMC分泌IL 10水平降低而使IFN γ水平升高。结论　在体外 ,加入适当的ILObjective To explore the IL-10 and IFN-γ Production in Supernatant of Systemic Lupus Erythematosus if Interleukin-12 added in vitro. Methods Using ELISA to measure the IL-10 and IFNγ levels in supernatant of PBMC from SLE patients and normal controls respectively, when IL-12 was added or not to the culture system.Results IL-12 up-regulated the IFN-γ level and down-regulated the IL-10 level in supernatant of SLE PBMC.Conclusion In vitro, IL-12 can rectify some abnormal cytokine phonotype in SLE.

关 键 词：红斑狼疮 系统性 细胞因子 夹心ELISA法 

分 类 号：R593.24[医药卫生—内科学]