皮质酮大鼠神经干细胞增殖分化的变化  

Changes of differentiation and proliferation of neural stem cells in rats treated with corticosterone

作　　者：高唱[1] 王景周[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内科,重庆400042

出　　处：《第三军医大学学报》2004年第17期1521-1523,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 2 716 52 )~~

摘　　要：目的　观察皮质酮 (corticosterone ,CORT)大鼠神经干细胞增殖分化的变化 ,探讨肾阳虚证与CORT大鼠神经干细胞增殖分化的联系。方法　成年雄性SD大鼠连续 14d皮下注射CORT ( 10mg·kg- 1 ·d- 1 ) ,采用免疫单标和双标方法检测成体大鼠神经干细胞及分化的功能细胞。给予CORT大鼠右归饮水煎剂灌胃 ,观察CORT大鼠神经干细胞增殖分化的变化。结果　与正常对照组相比 ,CORT大鼠大脑皮层、海马及下丘脑区BrdU阳性细胞数明显减少 ,BrdU +NF2 0 0 ,BrdU+NeuroD ,BrdU +GFAP免疫荧光双标细胞数也显著减少 (P <0 .0 1)。给予右归饮后CORT大鼠BrdU阳性细胞以及免疫荧光双标细胞数明显增加 (P <0 .0 1)。结论　CORT大鼠神经干细胞增殖分化能力显著下降 。Objective To explore the changes of differentiation and proliferation of neural stem cells in rats treated with corticosterone (CORT). Methods Adult SD rats were administered with CORT (10 mg·kg -1 ·d -1 ) by consecutive subcutaneous injection for 14 days. Single and double immunohistochemical stainings were used to identify the neural stem cells and differentiated neural cells in adult rats. Results Compared with that in the normal control group, the number of BrdU immunohistofluorescence positive cells and BrdU+NF200, BrdU+NeuroD, BrdU+GFAP double immunostaining positive cells in the cerebral cortex, hippocampus, and hypothalamus were markedly decreased ( P <0.01). After treatment of adult CORT rats with Yougui Yin , the number of BrdU and double immunostaining positive cells increased significantly ( P <0.01). Conclusion The differentiation and proliferation of neural stem cells in CORT treated rats were decreased significantly and Yougui Yin can reverse the abnormality of the differentiation and proliferation of neural stem cells in CORT treated rats.

关 键 词：右归饮 肾阳虚证 神经干细胞 增殖分化 

分 类 号：R256.59[医药卫生—中医内科学] R322.8[医药卫生—中医学]