良性前列腺增生组织上皮细胞的体外分离、纯化、培养及鉴定  

ISOLATION,PURIFICATION,CULTURE AND IDENTIFICATION OF HUMAN EPITHELIAL CELLS IN THE TISSUE OF BENIGN PROSTATIC HYPERPLASIA

作　　者：杨小丽[1] 卢少明[1] 庞友红[1] 付伟金[1] 李钟[1] 黄逢雨[1] 宣强[1] 

机构地区：[1]广西医科大学泌尿外科研究所,南宁530021

出　　处：《广西医科大学学报》2004年第4期469-471,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目 ( 3 9960 0 74)

摘　　要：目的 :分离、纯化、培养及鉴定良性前列腺增生组织上皮细胞。方法 :利用良性前列腺增生上皮细胞比重大的特点 ,将消化的良性前列腺增生组织 ,自然沉淀获得前列腺上皮细胞 ;并通过特定的培养基进一步纯化 ,选择性培养所获细胞 ;最后 ,通过形态观察、检测前列腺特异性抗原 (PSA)的 m RNA及其蛋白表达 ,鉴定上皮细胞。结果 :成功培养出良性前列腺增生上皮细胞。结论 :建立良性前列腺增生组织上皮细胞的分离。Objective:To separately culture prostate epithelial cells in the tissue of benign prostatic hyperplasia.Methods:Prostate epithelial cells were obtained by deposition ,then we used some special medium to get and reproduce more prostate epithelial cells.The prostate epithelial cells were identified by cell morphology,prostate specific antigen (PSA) expression of mRNA and protein.Result:We have cultured prostate epithelial cells. Conclusion: It is possible to develop a way to separately culture prostate epithelial cells.

关 键 词：良性前列腺增生 组织上皮细胞 体外分离 纯化 培养 鉴定 

分 类 号：R697.32[医药卫生—泌尿科学]