辐射花粉授粉和胚培养诱导产生黄瓜单倍体植株  

Recovery of cucumber (Cucumis sativus L.) haploid plants through pollination by irradiated pollens and embryo culture

作　　者：雷春[1] 陈劲枫[1] 钱春桃[1] 张永兵[1] 宋慧[1] 

机构地区：[1]南京农业大学园艺学院作物改良与种质创新国家重点实验室,南京210095

出　　处：《西北植物学报》2004年第9期1739-1743,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家高技术研究发展计划专项经费(2001AA241123;2002AA241251;2002AA207012);国家自然科学基金(30170644);教育部跨世纪优秀人才培养计划

摘　　要：以5个基因型的黄瓜(CucumissativusL.)为试材,通过γ射线辐射花粉授粉并结合胚培养,从3个基因型中获得了单倍体植株。与正常二倍体植株相比,单倍体植株生长缓慢,花器异常。研究发现辐射剂量、亲本基因型、授粉组合对座果率和单倍体产率有一定影响。Cucumber (Cucumis sativus L.) haploid plants were successfully recovered from three genotypes through pollination of five genotypes with γ-irradiated pollens and subsequent embryo culture.Comparing with the original diploid plants,the haploid plants grew slower with abnormal flower apparatus.The results obtained indicated that irradiation doses,parental genotypes,and pollination combinations had certain influences on both fruit setting and haploid production.

关 键 词：黄瓜 辐射花粉 胚培养 单倍体 

分 类 号：Q943.1[生物学—植物学]