儿童急性髓性白血病端粒酶活性的检测及临床意义  

Detection of telomerase activity in acute myeloid leukemia of children and its clinical significance

作　　者：文飞球[1] 曹励之[2] 陈亦欣 李博[1] 周克英[1] 李玉华[1] 陈霆[1] 

机构地区：[1]暨南大学医学院附属二院深圳市人民医院儿科,518020 [2]中南大学湘雅医院儿科,长沙410008 [3]深圳市人民医院肿瘤科

出　　处：《中国实用儿科杂志》2004年第9期549-551,共3页Chinese Journal of Practical Pediatrics

基　　金：广东省医学科研基金资助项目(项目编号 :A2 0 0 1616);教育部留学回国人员启动基金资助项目(教外司留[2 0 0 1] 3 45号 )

摘　　要：目的　探讨端粒酶活性与儿童急性髓性白血病 (AML)发病与发展的关系。方法　采用改良端粒重复序列PCR扩增 (PCR TRAP)方法检测 2 0 0 0年 11月至 2 0 0 3年 12月期间在深圳市人民医院和中南大学湘雅医院住院的 2 9例AML患儿骨髓单个核细胞化疗前后端粒酶活性的变化 ,并与 9例正常骨髓单个核细胞端粒酶活性进行比较。结果　初诊时 ,AML患儿骨髓细胞端粒酶活性 (35 5 5± 37 4 1)TPG ,较对照组 (2 36± 2 2 7)TPG明显增高 ,为对照组的 15倍 (P <0 0 1) ;化疗后 ,13例对治疗反应较好的患儿其端粒酶活性下降至 (2 6 6± 3 99)TPG ,其中10例≤ 1 5TPG。结论　AML患儿骨髓端粒酶活性明显上调 ,诱导缓解后显著降低。提示端粒酶活化与AML发展有关 。Objective To study telomerase activity in mononuclear cells (MNCs) of bone marrow (BM) specimens from pediatric patients with acute myeloid leukemia(AML) at diagnosis and after chemotherapy.For comparative analysis,MNCs from control donors were analyzed.Methods Telomerase in 29 cases of children with AML were evaluated using a modified telomeric repeat amplification protocol (TRAP) assay.Results At diagnosis,high telomerase activity was detected in MNCs from AML children (35 55±37 41TPG).This exceeded by 15 fold the activity in MNCs from control donors(2 36±2 27TPG,n=9) (P<0 01).After chemotherapy,telomerase activity in MNCs from the AML children decreased to (2 66±3 99) TPG,and 10 cases of the AML children less than 1 5TPG.Conclusion Telomerase is highly upregulated in BM but decreased after induction therapy;and it is correlated with remission in AML children.Our findings suggest that telomerase upregulating plays an important role in the development of AML and may be a useful predictor for prognosis in childhood leukemia.

关 键 词：白血病 端粒酶 儿童 

分 类 号：R72[医药卫生—儿科]