不同品系绍兴蛋鸭血清酯酶多态性比较研究  

Comparative Study on Serum Esterase Polymorphism Between Two Lines of Shaoxing Ducks

作　　者：张汤杰[1] 卢立志[2] 沈军达[2] 陈杰[1] 赵茹茜[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室 [2]浙江农科院畜牧所,杭州310021

出　　处：《中国家禽》2004年第18期10-12,共3页China Poultry

基　　金：国家自然科学基金资助项目(39770540)

摘　　要：实验对60日龄绍兴蛋鸭大蛋系(以蛋重为选育目标)和高产系(以产蛋数为选育目标)血清酯酶的多态性进行了比较测定。结果表明:60日龄绍兴鸭血清酯酶主要包括3个区域,即Es-1、Es-2和Es-3。Es-1区为鸭血清酯酶的主信号区,表现出明显的多态性,Es-2区未观察到明显的多态性,Es-3区为一至两条弱带(淡染),也未观察到明显的多态性。从260只60日龄绍兴鸭血清样本中,观察到Es-1有6种表现型:Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ和Ⅵ。绍兴蛋鸭大蛋系和高产系中以Ⅰ和Ⅱ居多,差异不显著,Ⅲ和Ⅳ在两品系鸭中表现出明显的差异,高产系Ⅳ型的表现频率显著高于大蛋系。The vertical polyacrylamide gel electrophoresis analysis was employed to compare the difference in the distribution patterns of esterase isozymes between two lines of 60-day-old duck hens,i.e.line selected for egg size (LSES)and line selected for egg production rate(LSEPR).The results showed that the serum esterase isozymes were mostly distributed in 3 regions:Es-1,Es-2 and Es-3.Es-1 was the most abundant region and showed clear polymorphism,while region Es-2 and Es-3 didn't show polymorphisms.6 phenotypes of esterase isozymes,Ⅰ?Ⅱ?Ⅲ?Ⅳ?Ⅴ?Ⅵ,were observed at region Es-1 from 260 ducks.Among 6 phenotypes of esterase isozymes in region Es-1,the frequency of typeⅠandⅡwas the highest.The frequency of type Ⅲ in region Es-1 was higher in LSES,yet the frequency of type Ⅳ in region Es-1 was higher in the LSEPR.

关 键 词：绍兴蛋鸭 血清酯酶 多态性 品系 

分 类 号：S834.89[农业科学—畜牧学]