绵羊Hbβ基因座频率分布的研究  

Study on frequency distribution at Hbβ locus of Sheep

作　　者：鲁生霞[1] 常洪[1] 杜垒[1] 角田健司 任战军[3] 孙伟[1] 徐琪[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]昭和大学医学部 [3]西北农林科技大学畜牧兽医学院

出　　处：《中国草食动物》2004年第5期3-5,共3页China Herbivores

基　　金：国家自然科学基金国际合作项目 (30 2 1 30 0 1 )

摘　　要：采用淀粉凝胶电泳和聚丙烯酰胺凝胶等电聚焦检测了在中心产区抽取的滩羊、同羊、小尾寒羊和湖羊 4个群体编码Hbβ基因座上的变异 ,搜集国内外 10个绵羊群体的相同资料 ,对Hbβ基因座频率分布进行分析。研究表明 :淀粉凝胶电泳只能检测出 3种表型 ,等电聚焦可以检测出 6种表型 ;近交是造成湖羊和同羊Hbβ座位遗传不平衡的主要原因 ;在高海拔 ( >3 0 0 0m )地区绵羊HbβA占优势 ,中、低海拔地区的绝大多数绵羊群体HbβB 占优势 ,HbβA 频率或HbβB 频率与海拔之间无线性变化关系。The variation of the locus encoding Hbβ was determined with Starch Gel Electrophoresis(SGE) and Polyacrylamide Gel Isoelectric Focusing(IEF) in 4 sheep populations,that is,Tan sheep,Tong sheep,Small Tailed Han sheep and Hu sheep,sampled in central areas of habitat.The same data of 10 sheep populations in China and other countries were collected in order to analyze frequency distribution of Hbβ locus. This study indicated that SGE could only examine 3 phenotypes while IEF could detect 6 phenotypes,inbreeding was the main reason resulting in genetic disequilibrium of Hbβ locus in Hu sheep and Tong sheep,HbβA was dominant in sheep raised at high altitude of more than 3 000 m ,HbβB was dominant in most sheep populations in the areas of middle and low altitude. The gene frequency of either of HbβA or HbβB didn't vary linearly with the altitude.

关 键 词：绵羊 Hbβ基因座 频率分布 淀粉凝胶电泳 聚丙烯酰胺凝胶等电聚焦 海拔地区 

分 类 号：S826.2[农业科学—畜牧学]