XRCC1基因多态性与慢性苯中毒易感性关系探讨  

Relation of genetic polymorphism of XRCC1 with risks of chronic benzene poisoning

作　　者：张忠彬[1] 曹多志 万俊香[1] 顾寿永[1] 金锡鹏[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]马鞍山钢铁公司医院劳研所

出　　处：《卫生研究》2004年第5期521-527,共7页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 2 71 1 1 3) ;国家 973项目 (No .2 0 0 2CB51 2 90 2 )

摘　　要：目的　探讨X线修复交叉互补基因 1(XRCC1基因 )的多态性与慢性苯中毒易感性的关系。方法　采用病例 -对照设计 ,以 15 2名苯中毒工人为病例组 ,15 2名接触苯而没有中毒表现的工人为对照组 ,应用聚合酶链反应 -限制性片段长度多态性分析技术 (PCR RFLP)检测XRCC1基因c.194、c.2 80和c.399位点的多态。结果　病例组中携带XRCC1c.194Arg Trp +Trp Trp基因型的个体的比例显著低于对照组 ,而携带XRCC1c .2 80Arg His+His His基因型的个体的比例显著高于对照组 ;携带XRCC1c.194Arg Trp +Trp Trp基因型的个体发生苯中毒的危险性较携带XRCC1c .194Arg Arg基因型的个体降低 1 6 7倍 (ORadj=0 6 0 ,95 %CI:0 37～ 0 97,P =0 0 39) ,而携带XRCC1c.2 80Arg His+His His基因型的个体发生苯中毒的危险性是携带XRCC1c.2 80Arg Arg基因型个体的 1 91倍 (ORadj =1 91,95 %CI:1 17～ 3 10 ,P =0 0 0 9)。结论　携带有XRCC1c .194Arg Trp +Trp Trp基因型的个体有较低的慢性苯中毒发病风险 ,而携带有XRCC1c.2 80Arg His+HisObjective To explore the relation between genetic polymorphisms in XRCC1 and risks of chronic benzene poisoning (BP). Methods A case-control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated. PCR-RFLP was applied to detect the single nucleotide polymorphisms (SNPs) on c.194, c.280 and c.399 of XRCC1 gene. Results The proportion of XRCC1c.194Arg/Trp+Trp/Trp genotypes in the case group was lower than that of the control group, while there was a higher proportion for XRCC1 C.280Arg/His+His/His in the case group. There was a 1.67-fold decreased risk of BP for individuals carrying XRCC1C.194Arg/Trp+Trp/Trp genotypes (ORadj=0.60, 95%CI:0.37-0.97, P=0.039) compared with subjects carrying Arg/Arg allele, and individuals carrying genotypes of XRCC1 C.280 Arg/His+His/His had a 1.91-fold increased risk of BP compared with these carrying the wild allele (ORadj=1.91, 95%CI:1.17-3.10, P=0.009). Conclusion The risk of BP for subjects carrying XRCC1c.194Arg/Trp+Trp/Trp genotypes may decrease while for individuals carrying XRCC1c.280Arg/His+His/His genotypes may increase.

关 键 词：苯中毒 X线修复交叉互补基因1 基因多态性 

分 类 号：R135.12[医药卫生—劳动卫生] Q343.1[医药卫生—公共卫生与预防医学]