纤维素类手性固定相高效液相色谱法拆分三唑类手性化合物  

HPLC Separation of Triazole Chiral Compounds on Cellulose Chiral Stationary Phases

作　　者：杨丽萍[1] 王立新[1] 徐艳丽[1] 钱宝英[1] 高如瑜[1] 

机构地区：[1]南开大学元素有机化学国家重点实验室元素有机化学研究所,天津300071

出　　处：《分析测试学报》2004年第5期25-28,共4页Journal of Instrumental Analysis

摘　　要：采用纤维素 -三 (3,5_二甲基苯基氨基甲酸酯 )手性固定相 (ChiralcelOD)和纤维素 -三 (4_甲基苯基甲酸酯 )手性固定相 (ChiralcelOJ) ,在正相高效液相 (N_HPLC)模式下 ,基线拆分了两个系列共13个结构类似的三唑类手性化合物 ,结果发现 ,当手性固定相(ChiralStationaryPhase,CSP)可以与溶质分子之间形成较强氢键时 ,ChiralcelOD的手性识别能力明显优于ChiralcelOJ ,当手性固定相 (CSP)与溶质分子之间不能或难于形成氢键时 ,两种CSP的手性拆分能力相似 ;提高流动相中极性改性剂的极性有利于手性化合物的拆分。在反相高效液相 (R_HPLC)模式下 ,共基线拆分了8个三唑类手性化合物 ,实验发现 ,OJ -CSP的手性拆分能力明显优于OD -CSP ,它们对对映体分子的选择性主要受CSP与溶质分子间的πUnder the normal_phase mode,Chiralcel OD and Chiralcel OJ were used as the chiral stationˉary phase(CSP),and a total of13triazole chiral compounds of2series were baseline resolved.Results indicated that Chiralcel OD showed better chiral recognition than Chiralcel OJ,if the chiral analyte has strong interaction with the CSP through hydrogen bonding.On the other side,if the analyte could not form strong hydrogen bond with the CSP,the chiral separation capacities of both CSPs are similar.Enanˉtiomeric separation could be improved by using a solvent of higher polarity.Under the reverse_phase mode,only eight triazole chiral compounds were baseline resolved.It was found that the performance of Chiralcel OD-R is obviously better than that of Chiralcel OJ-R.The degree of the chiral recognition of CSP depends strongly upon the π-π interaction between the analyte and CSP molecules in case of R-HPLC.

关 键 词：纤维素手性崮定相 高效液相色谱 三唑类手性化合物 氢键 手性识别 

分 类 号：O657.72[理学—分析化学] S482.2[理学—化学]