手性流动相添加剂法对两种手性化合物的直接拆分  

Separation of Enantiomers of Two Pesticides Usingβ-CD as A Chiral Mobile Phase Additive in High-performance Liquid Chromatography

作　　者：杨丽[1] 廖勇[2] 周志强[1] 江树人[1] 王鹏[1] 

机构地区：[1]中国农业大学应用化学系,北京100094 [2]中国科学院遗传与发育生物所,北京100101

出　　处：《分析测试学报》2004年第5期133-135,共3页Journal of Instrumental Analysis

摘　　要：以 β_环糊精为手性流动相添加剂 ,于C8 反相柱上建立了2种手性农药 (包括杀菌剂己唑醇和杀虫剂SR_生物丙烯菊酯 )对映体的高效液相色谱拆分方法。探讨了 β_环糊精浓度、流动相 pH、有机改性剂种类等因素对手性拆分的影响。结果表明 :在流动相为 β_环糊精水溶液、磷酸钠缓冲液(0.05mol/L)、乙腈、三乙胺(体积比50∶30∶20∶0.5)条件下 ,己唑醇对映体在pH为7.4 ,β_环糊精溶液浓度为7mmol/L时 ,SR_生物丙烯菊酯对映体在 pH为6.4 ,β_环糊精浓度为10.5mmol/L时得到最佳分离。A simple and effective HPLC method for the separation of enantiomers of two pesticides(hexaconazole and SR_bioallethrin)was developed by usingβ_cyclodextrin(β_CD)as a chiral mobile_ phase additive(CMPA)and a C 8 reversed_phase column.The effects ofβ_CD concentration,mobile_phase pH and organic modifier on chiral separation were studied.Excellent enantioseparation can be achieved for hexaconazole and SR_bioallethrin at pH7.4and6.4with aβ_CD concentration of7mmol/L and10.5mmol/L in a mobile phase consisting ofβ_CD,sodium phosphate buffer(0.05mol/L),acetonitrile and triethylamine in a volume ratio of50∶30∶20∶0.5.

关 键 词：对映体分离 反相高效液相色谱法 β-环糊精 手性流动相添加剂 手性农药 农药 

分 类 号：O657.72[理学—分析化学] S482.2[理学—化学]