亚甲蓝作为光谱探针研究对二甲氨甲基杯[8]芳烃与DNA间的作用  

Study of Interaction of p-(N,N′-Dimethylaminomethyl)-calix[8]arene with DNA Using Methylene Blue as Spectroscopic Probe

作　　者：李来生[1] 黄志兵[1] 刘旭[1] 谢冰[1] 王宇晓[1] 

机构地区：[1]南昌大学分析测试中心,南昌330047

出　　处：《应用化学》2004年第10期1011-1015,共5页Chinese Journal of Applied Chemistry

基　　金：江西省教委基金资助项目(JJ20037316);江西省自然科学基金资助项目(9920024)

摘　　要：利用紫外光谱法和荧光光谱法,以亚甲蓝(MB)为探针研究了水溶性对二甲氨甲基杯[8\]芳烃(简称CX8)与小牛胸腺DNA的相互作用,考察了溶液pH值、离子强度、常见有机溶剂及表面活性剂等对二者作用方式的影响,初步探讨了它们之间的作用机理,实验表明,大环化合物CX8与DNA之间主要存在较强的静电作用。通过Scatchard图解析证明,CX8可竞争DNA的MB静电结合位点。The interaction of water soluble p (N,N ′ dimethylaminomethyl)calix\arene(CX8) with calf thymus DNA has been studied by UV vis spectroscopy and fluorescence spectrophotometry using methylene blue(MB) as a probe. The effect of solution pH, ionic strength, common organic solvents and surfactants on the UV adsorption and fluorescence intensity were investigated. The results show that there is a strong electrostatic interaction between DNA and CX8. Scatchard curves indicated that the electrostatic interaction occurs between DNA and CX8, i.e. CX8 can compete for the electrostatic bonding sites of MB on DNA.

关 键 词：芳烃 小牛胸腺DNA 亚甲蓝 紫外光谱法 荧光探针 

分 类 号：O646[理学—物理化学]