茯苓多糖对受照射肿瘤细胞自由基的影响  

The Effect of PPS on the Free Radical of K562 Leukaemia Cell Line Esposed to γ-rays

作　　者：范雁[1] 吴士良[1] 徐爱华[1] 丁向明[1] 刘昌荣[1] 

机构地区：[1]苏州大学医学院生物化学与分子生物学教研室,江苏苏州215007

出　　处：《江苏大学学报（医学版）》2004年第30期194-195,217,共3页Journal of Jiangsu University：Medicine Edition

基　　金：国防科工委国防预研基金资助项目 (Y692 0 0 0 4) ;江苏省高校自然科学研究项目 (0 1KJB3 10 0 0 1)

摘　　要：目的 :观察茯苓多糖对受照射的肿瘤细胞及细胞培养上清液自由基相关指标的影响。方法 :加不同剂量茯苓多糖的K5 6 2白血病细胞在60 Coγ射线 15Gy照射后 ,立即收集上清液和细胞样本检测自由基的变化。结果 :受照后细胞培养上清液中活性氧 (ROS)含量和丙二醛 (MDA)含量有明显的升高 ,加药后有所降低但仍高于未照射组 (P≤0 0 5 ) ,超氧化物歧化酶 (SOD)浓度在受照射及加入多糖后无明显变化规律 ;细胞中活性氧含量及MDA在受照射后增加 ,加入茯苓多糖后活性氧含量仍继续升高 ,而MDA有所降低但仍明显高于未照射组 ,SOD活性在受照射后有所下降 ,加药后SOD活性有所升高但仍低于未照射对照组 (P≤ 0 0 5 )。结论 :茯苓多糖对γ射线照射引起肿瘤细胞中自由基活性的增高无显著影响 ,由此推测茯苓多糖对正常细胞辐射防护作用的同时基本不会影响放疗的效果。Objective: To observe the effect of Pachyman polysaccharides (PPS) on the free radical of irradiated Leukaemia cells and their culture mediums. Methods: The cells in the control group and PPS (50 mg/L,100 mg/L,200 mg/L)groups were radiated with 15Gy γ-rays,then reactive oxygen species(ROS),superoxide dismutase(SOD) and malonyldialdehyde(MDA) of the cells and their culture mediums were examined as soon as we can. Results: The content of ROS and MDA in culture mediums were enhanced obviously after radiation. While they decreased somewhat in PPS groups,they still exceeded the non-irradiated control group( P ≤0 05).The avidity of SOD did not show any significant changes after irradiation or to be added to PPS. In cells,the content of ROS was enhanced,and it continued to increase after to be added to PPS. The avidity of SOD was lower after irradiation. While it increased somewhat in PPS groups,it was still less than the non-irradiated control group( P ≤0 05). Conclusion: There was no notable effects of PPS to Hydroxyl free radical’s increasing caused by radiation in tumor cells,and we supposed that PPS was unable to influence the effects of radiotherapy when it exerted the radioprotection to normal cells.

关 键 词：茯苓多糖 辐照 肿瘤细胞 自由基 

分 类 号：R282.7[医药卫生—中药学]