骨髓间充质干细胞移植对大鼠心肌梗死后细胞凋亡的作用  

Effect of mesenchymal stem cells transplantation on the apoptosis after rat myocardial infarction

作　　者：唐滔[1] 胡建国[1] 杨进福[1] 周新民[1] 杨一峰[1] 尹邦良[1] 喻本桐[1] 

机构地区：[1]中南大学湘雅二医院胸心外科,长沙410011

出　　处：《中南大学学报（医学版）》2004年第3期274-278,共5页Journal of Central South University ：Medical Science

基　　金：中国博士后研究基金 (中博金「2 0 0 0」31号 ) ;美国中华医学基金项目 (CMB :99 698) ;湖南省社会发展计划项目 (0 3 PCY2 0 1 2 )

摘　　要：目的 :探讨骨髓间充质干细胞心肌移植对大鼠心肌梗死区细胞凋亡的影响。方法 :取大鼠骨髓间充质干细胞进行体外培养、增殖和标记 ,同时以液氮冷冻左室游离壁建立心肌梗死模型 ,4周后分别将 2× 10 6个细胞悬液或冷D Hanks液注入心肌梗死区数个不同部位。于移植后 1周、2周和 4周依次获取心肌梗死区标本。随机于第 2周实验组与对照组中各抽取两个标本进行透射电镜检查 ,凋亡细胞计数 ,RT PCR检测所有标本bcl 2mRNA的表达。结果 :部分心肌细胞呈调亡细胞初期形态改变 ,实验组和对照组调亡指数分别为 0 .0 2和 0 .0 8。bcl 2mRNA在术后第1,2及 4周的表达水平实验组与对照组相比均增加 (P <0 .0 1) ,且随着时间的推移其表达量两组均呈迅速降低的趋势 (P <0 .0 1)。结论 :骨髓间充质干细胞心肌移植可促进心肌梗死区Bcl 2蛋白表达 ,并减轻大鼠心肌梗死后细胞凋亡。Objective To investigate the effect of cardiac transplanting of mesenchymal stem cells on the apoptosis after rat myocardial infarction.Methods Wistar inbred rats were used to stimulate autograft. Harvested rat mesenchymal stem cells were cultivated, proliferated and labeled in vitro. At the same time, myocardial infarct models were set up using liquid nitrogen to cryoinjury the free wall of the left ventricule.Four weeks later, 2×10 6 mesenchymal stem cells or cold D-Hanks liquid were injected into several different points of infarct area. After 1, 2 and 4 weeks, specimens from infarct area were obtained in order. Then the number of apoptosis was counted under scanning electron microscope in every 2 specimens selected at random from the experimental group and the reference group within 2 weeks. The bcl-2 mRNA expression of all rats was assayed by RT-PCR.Results The observation of transmission by electron microscope showed that part of the myocardial cells became apoptosis in the initial stage in ultramicrostructure. Apoptosis ratio was 0.02 and 0.08 in the experimental group and the reference group respectively,which demonstrated that mesenchymal stem cells could relieve apoptosis after rat myocardial infarctation. The levels of bcl-2 mRNA expression in 1, 2, and 4 weeks were all obviously promoted in the experimental group (3.235±0.126,1.152±0.021,0.798±0.016)than those in the reference group (2.695± 0.084 , 0.537 ±0.022,0.579±0.019) (P<0.01), and quickly decreased with time.Conclusion Mesenchymal stem cells transplantation can promote the expression of bcl-2 mRNA in the infarct area and relieve apoptosis after rat myocardial infarction.

关 键 词：骨髓 间充质干细胞 细胞移植 大鼠 心肌梗死 细胞凋亡 Bcl-2 血管新生 细胞计数 

分 类 号：R542.22[医药卫生—心血管疾病] R-332[医药卫生—内科学]