蒿甲醚与瑞香素伍用对感染伯氏疟原虫小鼠的治疗作用  

Additive Therapeutic Effect of a Combination of Artemether and Daphnetin against Plasmodium berghei in Mice

作　　者：郭俭[1] 倪奕昌[1] 吴嘉彤[1] 王琴美[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第3期164-166,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：青蒿素科技基金资助课题 (2 0 0 2 1 0 )~~

摘　　要：目的　研究蒿甲醚与瑞香素伍用 (A +D)对感染伯氏疟原虫ANKA株小鼠的疗效及其联合作用方式。　方法　按“四天抑制法”d0 感染 ,d0 ～d3 给药 ,每天 1次 ,d4涂薄血膜检查 ,并计算d4减虫率及各药丰数有效置 (ED50 ) ,用等效应图解法分析A +D的合并作用。　结果　蒿甲醚 0 .4mg/(kg·d)× 4d的d4减虫率与对照组相比差异无显著性 ;[A0 .4mg/(kg·d) +D7.7mg/(kg·d) ]× 4d的抗疟效果提高 ,与对照组相比差异有显著性 (P <0 .0 5 )。A +D各组的ED50 均低于单用药组 ;不同配伍比例的R值皆大于 0 .4,小于 2 .7( 0 .4<R <2 .7)。　结论　蒿甲醚与瑞香素伍用治疗感染伯氏疟原虫小鼠能提高抗疟疗效 ,且两药合并呈相加作用。Objective To investigate the therapeutic effect of a combination of artemether and daphnetin against Plasmodium berghei ANKA strain in mice. Methods Groups of P. berghei infected mice were treated with various oral doses of artemether and daphnetin according to “4-day suppress assay”. Thin blood smears were made on the fifth day after inoculation of parasites and the parasitemia reduction rate was calculated. The ED 50 values obtained were plotted on isobolograms. A combined action of artemether and daphnetin was assessed. Results Artemether 0.4mg/(kg·d)×4d exhibited no detectable antimalarial effect, while artemether 0.4mg/(kg·d)×4d combined with daphnetin 7.7 mg/kg.d×4d showed potent antiparasile efficacy. The ED 50s of artemether in combination with daphnetin were lower than that of single artemether or daphnetin. The R-values were higher than 0.4, but lower than 2.7. Conclusion The combination of artemether with daphnetin showed an additive antiparasile effect.

关 键 词：蒿甲醚 瑞香素伍 感染伯氏疟原虫 小鼠 药物疗法 药物配伍 

分 类 号：R382.31[医药卫生—医学寄生虫学]