乌鲁木齐地区人巨细胞病毒临床分离株PCR-RFLP分析研究  

Study on clinical isolates of human cytomegalovirus found in Urumqi by restriction fragment length polymorphism of PCR products

作　　者：杨学磊 加娜尔 孙荷 杜文慧 

机构地区：[1]新疆自治区儿科研究所,乌鲁木齐830001

出　　处：《中华实验和临床病毒学杂志》2004年第2期168-171,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　通过对人巨细胞病毒临床分离株基因结构的分析 ,了解乌鲁木齐地区人巨细胞病毒 (HCMV)的分子流行病学情况。方法　分别扩增 2 8株HCMV临床分离株的 3个相对保守基因 :DNA聚合酶基因 (pol) ,糖蛋白H基因 (gH)和主要即刻早期蛋白基因 (MIE)片段并进行限制性片段长度多态性 (PCR RFLP)分析。结果　非流行病学相关毒株限制性图谱差异较大 ,流行病学相关毒株如母婴配对株限制性图谱有很大的相似性。 8对母婴配对HCMV毒株 ,4对限制性图谱一致 ,4对存在差异。结论　本地HCMV分离株基因变异广泛存在。PCR RFLP分析可揭示HCMV感染的传播方式 ,可用于病毒的分子流行病学研究。Objective The study was designed to investigate the status of molecular epidemiology of HCMV in Urumqi through genetic comparison of clinical isolates. Methods DNA sequences of 2.0-2.6 kb were amplified by polymerase chain reaction from three relatively conservative gene regions (DNA polymerase,glycoproteins H,and major immediate-early antigen) of 28 clinical HCMV strains and then were analysed by restriction enzymes. Results The restriction patterns of the clinical isolates which did not have relation in epidemiology were greatly different,but the patterns of the clinical isolates related in epidemiology such as strains paired in mother and infant were quite similar. Of eight mother and infant pairs,from whom HCMV were isolated,four pairs showed identity of restriction profiles within each pair for all three amplified regions,four pairs showed differences between mother and infant. Conclusion These results confirm the high degree of genetic variability among cytomegalovirus strains in Urumqi. Analysis of PCR-RFLP can indicate transmission of HCMV infection and facilitate its molecular epidemiologic studies.

关 键 词：乌鲁木齐地区 人巨细胞病毒 临床分离株 PCR-RFLP 基因结构 流行病学 

分 类 号：R373[医药卫生—病原生物学]