Cloning and Characterization of the fecC Gene Necessary for Optimal Growth under Iron-Deficiency Conditions in the Cyanobacterium Anabaena sp. PCC7120  

作　　者：xuwen-liang LIUYong-ding ZHANGCheng-cai LIJuan 

机构地区：[1]CollegeofLifeSciences,CentralChinaNormalUniversity,Wuhan430079,Hubei,China [2]InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,Hubei,China [3]CollegeofLifeSciencesandTechnology,HuazhongAgriculturalUniversity,Wuhan430070,Hubei,China [4]CollegeofLifeSciences,CentralChinaNormalUniversity,Wuhan430079,Hubei,China

出　　处：《Wuhan University Journal of Natural Sciences》2004年第4期498-502,共5页武汉大学学报（自然科学英文版）

基　　金：SupportedbytheNationalNaturalSciencesFoundationofChina(30 0 70 1 54) ;theFrontierScienceProjectsProgramoftheInstituteofHydrobiology;theChi neseAcademyofSciences (2 2 0 31 6) ;StateKeyProjectonCyanobacterialBloomControlinLakeDianchi(K99 0 5 35 0 1

摘　　要：ThefecC gene encoding a putative iron (III) dicitrate transporter was cloned from nitrogen-fixing cyanobacteriumAnabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO 3 ? , NH 4 + or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wildtype strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that thefecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed thatfecC gene product is required for optimal growth under iron-deficient conditions inAnabaena sp. PCC 7120. Key words Anabaena sp. PCC 7120 - fecC - iron deficiency - photosynthetic properties - expression CLC number Q 933 Foundation item: Supported by the National Natural Sciences Foundation of China (30070154), the Frontier Science Projects Program of the Institute of Hydrobiology, the Chinese Academy of Sciences (220316), State Key Project on Cyanobacterial Bloom Control in Lake Dianchi (K99-05-35-01)Biography: XU Wen-liang (1974-), male, Ph. D, research direction: molecular genetics of cyanobacteria.ThefecC gene encoding a putative iron (III) dicitrate transporter was cloned from nitrogen-fixing cyanobacteriumAnabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO 3 ? , NH 4 + or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wildtype strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that thefecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed thatfecC gene product is required for optimal growth under iron-deficient conditions inAnabaena sp. PCC 7120. Key words Anabaena sp. PCC 7120 - fecC - iron deficiency - photosynthetic properties - expression CLC number Q 933 Foundation item: Supported by the National Natural Sciences Foundation of China (30070154), the Frontier Science Projects Program of the Institute of Hydrobiology, the Chinese Academy of Sciences (220316), State Key Project on Cyanobacterial Bloom Control in Lake Dianchi (K99-05-35-01)Biography: XU Wen-liang (1974-), male, Ph. D, research direction: molecular genetics of cyanobacteria.

关 键 词：Anabaena sp. PCC 7120 fecC iron deficiency photosynthetic properties expression 

分 类 号：Q945.1[生物学—植物学]