同型半胱氨酸对家兔组织及血浆金属硫蛋白含量的影响  

Effects of homocysteine on tissues and metallothionein contents of plasma in rabbits

作　　者：林波 聂晓兰[1] 张永雪 

机构地区：[1]河南大学医学院病理生理教研室 [2]开封市95936部队医院

出　　处：《河南大学学报（医学科学版）》2004年第2期12-14,共3页

摘　　要：目的 :观察高同型半胱氨酸 (Hcy)血症时家兔组织及血浆金属硫蛋白 (MT)的变化。 方法 :家兔皮下注射Hcy复制高Hcy血症模型 ,测定肝、心、肾组织及血浆MT和丙二醛 (MDA)含量。 结果 :Hcy组家兔肝、心、肾组织及血浆MT水平较对照组分别升高 2 30 % (P <0 .0 1 )、1 30 % (P <0 .0 1 )、5 4 .8% (P <0 .0 1 )和 85 % (P <0 .0 1 ) ,而丙二醛水平则分别高 1 0 0 % (P <0 .0 1 )、71 % (P <0 .0 1 )、5 0 % (P <0 .0 1 )和 1 1 0 % (P <0 .0 1 )。注射Hcy同时用叶酸处理动物 ,肝、心和肾组织MT含量较Hcy组分别降低 36 % (P <0 .0 1 )、33% (P <0 .0 1 )、2 9%和 36 % (P <0 .0 1 ) ,丙二醛含量分别降低 5 2 % (P <0 .0 1 )、4 1 % (P <0 .0 1 ) 4 5 % (P <0 .0 1 )和 2 9% (P <0 .0 1 )。结论Objective: To observe the changes of metallothionein (MT) in various tissues and plasma in rabbits during hyperhomocysteinemia Methods: Hyperhomocysteinemia modlel of rabbit was made by intradermal injection of homocysteine. The levels of MT and malondialdehyde (MDA) were measured in the tissues of liver, heard, kidney and plasma of rabbit .Results: Compared with control group, the MT levels in liver, heat, kidney and plasma in hcy group increased 230%( P <0.01), 130%( P <0.01), 55%( P <0.01)and 85%( P <0.01).The MDA levels in liver, heart, kidney and plasma in hcy group increasd 100%( P <0.01), 71%( P <0.01), 50%( P <0.01)and 110%( P <0.01)respectively . When Hcy was injected and at the same time, the animals' liver, heart and kidney tissues were processed with folacin, the MT contents in Hcy group respectively decreased 36%( P <0.01),33%( P <0.01), 29% and 36%( P <0.01), and the MDA contents decreased 52%( P <0.01)、41%( P <0.01)45%( P <0.01)and 29%( P <0.01).Conclusion: MT formation in rabbits' tissues and plasma induced by homocysteine may be related to oxidative stress.

关 键 词：同型半胱氨酸 家兔 血浆 金属硫蛋白 丙二醛 肝组织 心组织 肾组织 高同型半胱氨酸血症 

分 类 号：R589[医药卫生—内分泌]