发囊基因芯片法和血样单克隆抗体法在HLA-Ⅱ类分型中的比较研究  

A comparative study of hair microarray and blood monoclonal antibody in HLA-Ⅱ typing

作　　者：黄伟[1] 程敦秀[1] 王大卫[1] 汪芯[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,武汉430030

出　　处：《中华器官移植杂志》2004年第4期194-196,共3页Chinese Journal of Organ Transplantation

基　　金：国家科技部<重大基础研究前期研究>专项基金资助项目( 2 0 0 2CCD0 150 0 )

摘　　要：目的　同步双盲法研究单克隆抗体法和基因芯片法在HLA分型中的准确性、重复性和实用性。方法　研究样本 4 6份 ,单克隆抗体法采用一步法单抗分型技术 ,基因芯片法采用寡核苷酸芯片技术。结果　 4 6份标本的单克隆抗体法和基因芯片法分型成功 ,对有差异的结果进行基因芯片法复查 ,重复率 10 0 %。单克隆抗体法耗时 1.5h ,基因芯片法耗时 3.5h。基因芯片法的漏检率低于单克隆抗体法。两种方法主要在对DR2 (DR15 ,DR16 )的检测结果上不一致。结论　目前HLA Ⅱ类抗原的分型 ,尤其是临床大样本分型 ,可以采用单克隆抗体分型技术进行快速分型。对于要求精细配型的骨髓移植 ,应该采用基因芯片分型或其他DNA分型技术。对于单克隆抗体分型结果可疑的样本应用基因芯片分型确认。Objective A double-blind synchro-method was carried out to evaluate the reliability,reproducibility and practicability of microarray typing in comparison with monoclonal antibody in identifying HLA-Ⅱ alleles.Methods In 46 samples,HLA classⅡ was typed by hair oligonucleotide microarray typing and peripheral blood one-step monoclonal antibody typing respectively.Results All samples was successfully typed by microarray. Monoclonal antibody typing for HLA-Ⅱ was rapid,taking 1.5 h ,but microarray typing taking 3.5 h . Examination by both of the 2 methods indicated high rates of misdiagnoses by monoclonal antibody typing. Diagnostic discrepancy occurred chiefly in DR15 and DR16.Conclusion HLA class Ⅱ typing was performed by using monoclonal antibody typing technique. Microarray typing or other DNA typing techniques were used to refined typing such as bone marrow transplantation. Doubtful samples by monoclonal antibody typing can be typed by microarray typing.

关 键 词：发囊基因芯片法 血样单克隆抗体法 HLA-Ⅱ类 基因分型 HLA分型 

分 类 号：R446.9[医药卫生—诊断学] R446.6[医药卫生—临床医学]