11,12-EET对未成熟离体兔心的长时间保存作用  

Prolonged myocardial protection of 11,12-epoxyeicosatrienoic on immature isolated rabbit hearts

作　　者：周明阳[1] 吴清玉[1] 钟星[2] 龙村[3] 温复兴[3] 覃筱燕[4] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院心外科,北京100037 [2]中国医学科学院肿瘤研究所 [3]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院麻醉与体外循环研究室,北京100037 [4]中央民族大学生物化学系

出　　处：《中华器官移植杂志》2004年第4期203-205,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　观察 11,12 EET(11,12 epoxyeicosatrienoicacid)液对未成熟兔离体心脏的保护作用。方法　将 4 8只未成熟离体兔心按随机分配原则分成对照组 (2 4只 )和EET组 (2 4只 ) ,对照组保存心脏采用St.ThomasNo .2液为停搏液及保存液 ,EET组采用St.ThomasNo .2液 +11,12 EET为停搏液及保存液 ,分别保存心脏 8h、16h、2 4h。利用非循环式Langendorff灌注装置测定各组心脏保存前及保存后再灌注 30min(37℃ )的左心室变化压 (LVDP)、舒张末期压 (LVEDP)、最大压力变化速率 (+dp/ptmax)和冠状动脉流量 (CBE) ,并测定心律失常评分、心肌肌酸激酶 (CK)、乳酸脱氢酶(LDH)含量及观察心肌超微结构的改变等指标。结果　 (1)EET组各保存时间的心功能恢复率、心肌水肿程度、心律失常评分均好于相应对照组 ;CK、LDH及心肌超微结构的改变亦明显优于对照组。(2 )心脏保存 16h后 ,EET组的心功能恢复率、心律失常评分基本接近心脏保存之前的测定值 ,而对照组则明显减低。 (3)心脏保存 2 4h ,EET组心脏全部复跳 ,对照组 5只心脏不能复跳。结论　St.ThomasNo .2液中加入 11,12 EET可延长对未成熟兔离体心脏的保存时间并增强其保存效果。Objective To observe the protective effect of 11,12-epoxyeicosatrienoic acid(11,12-EET)on immature isolated rabbit hearts.Methods Forty-eight isolated immature rabbit hearts were randomly assigned to two groups: Control group,the hearts were arrested with St.Thomas No.2 solution and stored in the same solution ( n =24);EET group,the hearts were arrested with St.Thomas No.2 plus 11,12-EET solution and stored in the same solution ( n =24). All rabbit hearts were stored for 8,16 and 24 h with 4 ℃ hypothetmia, and underwent 30 min reperfusion ( 37 ℃ ). On the Langendorff perfusion apparatus,left ventricle developed pressure (LVDP),left ventricle end-diastolic pressure (LVEDP),+dp/pt max ,coronary blood effluent (CBE) and arrhythmias score were measured before and after ischemia. Myocardial water content,the value of creatine kinase (CK) and lactic dehydrogenase (LDH) were also measured,and myocardial ultrastructure observed. Results Postischemic recovery of myocardial function and myocardial edema were significantly better in EET group at different time points. The changes of arrhythmias score,CK,LDH and myocardial ultrastructure in EET group were superior to those in control group. After the hearts were preserved for 16 h ,the recovery of myocardial function and arrhythmias score in EET group were basically close to the measured values before heart preservation,while those in control group were significantly decreased. After the hearts were stored for 24 h ,all hearts in EET group beated again during reperfusion,but 5 hearts in control group could not beat anymore.Conclusion Addition of 11,12-EET into the St.Thomas No.2 cardioplegic solution could prolong the storage time and enhance the myocardial protective effect to the isolated immature hearts.

关 键 词：EET 离体心脏 器官保存液 心脏停搏 心功能 

分 类 号：R654.2[医药卫生—外科学]