高效液相色谱法假尿苷次黄嘌呤含量测定  

Determination on Concentration of Pseudouridine and Hypoxanthine in the Human Serum by HPLC

作　　者：王景萍[1] 李杰[1] 周丽莉[1] 郭嬿[1] 王玲 

机构地区：[1]河南省肿瘤医院,郑州市450008 [2]河南省肿瘤研究所,450003

出　　处：《医药论坛杂志》2004年第10期21-22,共2页Journal of Medical Forum

摘　　要：目的　建立一种同时检测人血清中假尿苷、次黄嘌呤含量的高效液相色谱方法。方法　色谱条件 :Spherigel-C18色谱柱 ;流动相 :含 0 2 %乙腈的 0 0 2mol/LKH2 PO4缓冲液 ;流速 :1ml/L ;UV :2 5 4nm。结果　本法假尿苷线性范围为 1mg/L～ 16mg/L ,相关系数r =0 9986 ,平均回收率 96 % ,最低检测浓度 0 5mg/L ;次黄嘌呤线性范围为 1mg/L～ 2 0mg/L ;相关系数r =0 990 2 ;平均回收率 97% ;最低检测浓度为 0 3mg/L。 结论　本法具有样品处理简单、稳定性高等特点 ,经临床验证具有较好的实用性。Objective To establish a method for the determination of concentration of pseudouridine and hypoxanthine in the human serum.Methods The concentration of Pseudouridine and hypoxanthine was assayed on a Spherigel-C 18 column with a mobile phase consisting of acetonitrile:0.02mol/L KH 2PO 4(0.2%) at a flow rate of 1ml/min and detected at UV 254nm.Results The calibration curve of pseudouridine was linear over the range of 1mg/L～16mg/L(r=0.9986),The average recovery of Pseudouridine was 96%,the limit of quantification was 0.5mg/L.The calibration curve of hypoxanthine was linear over the range of 1mg/L～20mg/L(r=0.9902),the average recovery of hypoxanthine was 97%,the limit of quantification was 0.3mg/L.Conclusion This method possessed the features of simple sample treatment and high stability.Clinical application demonstrated that this method had a better practicality.

关 键 词：高效液相色谱法 假尿苷 次黄嘌呤 含量测定 血清 

分 类 号：R446.112[医药卫生—诊断学]