光和暗条件下低温对黄瓜和水稻叶绿素蛋白质复合体的影响  

THE EFFECT OF CHILLING STRESS ON CHLOROPHYLLPROTEIN COMPLEXES OF RICE AND CUCUMBER SEEDLINGS IN THE LIGHT AND DARK

作　　者：刘鸿先[1] 王以柔[1] 李晓萍[1] 郭俊彦[1] 

机构地区：[1]中国科学院华南植物研究所,广州510650

出　　处：《广西植物》1993年第2期174-179,共6页Guihaia

基　　金：国家自然科学基金

摘　　要：本试验以黄瓜和水稻幼苗为材料,研究了光照和黑暗条件下低温对植物叶绿素蛋白质复合体的影响。SDS—PAGE电泳结果表明:5℃及12h 280μmol m^(-2)S^(-1)处理2d,Chl-蛋白质复合体的降解明显大于5℃暗低温处理;低温与光照对P700-CPa_1的影响大于LHCP。叶绿素荧光测定表明;5℃及12h 280μmol m^(-2)s^(-1)的处理对PSⅡ的影响亦大于暗低温处理。由此认为:低温与光对植物叶绿体的PSⅠ和PSⅡ都有明显的影响,其机理可能与常温下高光强引起的光抑制相类似;不同的是低温下中等光强就能引起光抑制。因此,在光照低温下往往加剧植物冷害的发生。In this work we used cucumber and rice seedlings as experimentalmaterials to study the changes of chlorophyll-protein complexes in leaves afterchilling treatment under dark and light. Result indicated that the chlorophyll-protein complexes were decreased in the seedlings exposed to 5℃ for 2 days. Theleaves treated with 5℃ and 280 μ mol m^(-2)s^(-1) was decreased more than that of theleaves under dark at same temperature. Effect of chilling and light interaction onP700--CPa_1 was great, while it was less on LHCP. PSⅡ activity was monitoredby chlorophyll fluorescence emission. It declined rapidly at 280 μmol m^(-2)s^(-1) PFD,but it decreased slowly at dark under same temperature. Therefore, we conclude that this injury mechanism was similar to photoinhibi-tion by strong light intensity, while photoinhibition induced by chilling tempera-ture could carry out even in a moderate photon irradiance. The interaction of lowtemperature and light did, however, result an increase at chilling injury.

关 键 词：光 黄瓜 低温 水稻 叶绿素 蛋白 

分 类 号：S311[农业科学—作物栽培与耕作技术]