In vitro anti-coxsackievirus B_3 effect of ethyl acetate extract of Tian-hua-fen  

作　　者：Zhen-HongLi Bao-MingNie HongChen Shu-YunChen PingHe YangLu Xiao-KuiGuo Jing-XingLiu 

机构地区：[1]DepartmentofMicrobiologyandParasitology,ShanghaiSecondMedicalUniversity,Shanghai200025,China

出　　处：《World Journal of Gastroenterology》2004年第15期2263-2266,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by Project of National Nature Science Foundation of China,No.39970691 and Project from Education Comment of Shanghai,China,No.2000B04

摘　　要：AIM: To investigation the anti-coxsackievirus B3 (CVB3m)effect of the ethyl acetate extract of Tian-hua-fen on HeLacells infected with CVB3m.METHODS: HeLa cells were infected with CVB3m and thecytopathic effects (CPE) were observed through light microscope and crystal violet staining on 96-well plate and A600 was detected using spectrophotometer. The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A600.RESULTS: The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration.Consistent results were got through these two methods.We also investigated the mechanism of its anti-CVB3m effectand the results indicated that the extract represented an inhibitory effect through all the processes of CVB3m attachment, entry, biosynthesis and assemble in cells.CONCLUSION: The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significantprotectiveeffect on HeLa cells infected with CVB3m in a dose-dependent manner and this effect exists through the process of CVB3mattachment, entry, biosynthesis and assemble in cells,suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.AIM:To investigation the anti-coxsackievirus B_3(CVB_(3m)) effect of the ethyl acetate extract of Tian-hua-fen on HeLa cells infected with CVB_(3m). METHODS:HeLa cells were infected with CVB_(3m) and the cytopathic effects(CPE)were observed through light microscope and crystal violet staining on 96-well plate and A_(600) was detected using spectrophotometer.The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A_(600). RESULTS:The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration. Consistent results were got through these two methods. We also investigated the mechanism of its anti-CVB_(3m) effect and the results indicated that the extract represented an inhibitory effect through all the processes of CVB_(3m) attachment,entry,biosynthesis and assemble in cells. CONCLUSION:The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significant protective effect on HeLa cells infected with CVB_(3m) in a dose-dependent manner and this effect exists through the process of CVB_(3m) attachment,entry,biosynthesis and assemble in cells, suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.

关 键 词：柯萨奇病毒B3 乙基醋酸纤维 试管 天花粉 CVB3M 病毒感染 

分 类 号：R512.4[医药卫生—内科学]