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机构地区:[1]广西植物研究所,桂林541006
出 处:《广西植物》1993年第2期144-145,共2页Guihaia
摘 要:报道6个香蕉品种的小茎尖离体繁殖无病苗中芽产生的细胞学观察。小茎尖在改良MS附加BA 3.0 mg/l的培养基上培养,5天后可以看见外植体基部膨大,10天后叶原基伸长、转绿,20天后长出叶子形成苗。20—30天左右,所形成苗的两侧表皮下的薄壁细胞转化为分生细胞,形成芽原基,继续分化形成芽,一般芽数为2—3个。This paper reports the cytological observation on bud formation byshoot-tip culture on modified MS medium containing 3.0mg/l BA. After five daysof culture, the base of the explants began to expand. Ten days later, the leafprimordium grew and became longer and changed to green in color. After twentydays it differentiated to leaves and shoot. After 20-30 days, the parenchymatouscells beneath the epidermal on both sides of the shoot transformed into meristema-tic cells, and bud were formed, then continued to differentiate into 2--3 buds.
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