兔软骨终板退变过程超微结构的观察  

Observation of ultrastructure on the degeneration of the cartilage end plate in rabbits

作　　者：刘斌[1] 瞿东滨[1] 金大地[1] 陆地[2] 安连兵[3] 

机构地区：[1]第一军医大学南方医院脊柱骨病外科,广州510515 [2]昆明医学院解剖学教研室,昆明650031 [3]第一军医大学中心实验室电镜室,广州510515

出　　处：《中国临床解剖学杂志》2004年第4期398-401,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30000168);广东省自然科学基金(001105)

摘　　要：目的:研究兔软骨终板退变过程中的超微结构变化。方法:将18只新西兰白兔随机均分为实验组和对照组,实验组通过切除兔腰椎棘上、棘间韧带,咬除部分关节突关节,分离椎旁肌造成腰椎软骨终板退变模型,分别于术后12、24、36周对腰椎摄X线片,并行扫描电镜观察软骨终板超微结构的变化。结果:X线片示软骨终板随时间的延长而逐渐钙化,且实验组较对照组钙化明显;扫捕电镜示实验组软骨终板随时间延长胶原纤维逐渐变细、排列紊乱、出现裂隙,蛋白多糖丢失加剧,且上述变化较对照组显著。结论:椎间失稳可造成软骨终板退变,导致软骨终板基质的破坏。To study the changes of the ultrastructure on the degeneration of the cartilage end plate (CEP). Methods: An experiment model of lumbar CEP degeneration was established in 18 rabbits by resection of all lumbar supraspinous and interspinous ligaments, excision of parts of the zygapophysial joints and detachment of the posterior paravertebral muscles from lumbar vertebraes. After the operation, the lumbar spine was examined by X-ray in 12, 24 and 36 weeks, respectively. The changes of the ultrastructure of CEP were also observed by scanning electron microscope during this process. Results: X-ray graph showed that the CEP calcified gradually with time increasing, and experiment groups calcified more obviously than those of the control groups. The scanning electron microscope showed that the collagen fibers tended to be thin, disorder and split as the time passed, and the content of proteoglycan lost more severely. All these changes were more obvious in the experiment groups. Conclusions: Mechanical instability of the lumbar spine could result in the degeneration of CEP and lead to the breakdown of its extracellular matrix.

关 键 词：腰椎 退变 软骨终板 超微结构 

分 类 号：R339.4[医药卫生—人体生理学]