血管紧张素Ⅰ转换酶基因多态性与2型糖尿病肾病相关性研究  

Association between angiotensin Ⅰ converting enzyme gene insertion/deletion polymorphism and Type 2 diabetes nephropathy

作　　者：李鸣[1] 刘丽梅[1] 郑泰山[1] 张蓉[1] 李杰[1] 项坤三[1] 

机构地区：[1]上海交通大学附属上海市第六人民医院内分泌代谢科,上海市糖尿病研究所200233

出　　处：《上海医学》2004年第7期457-459,共3页Shanghai Medical Journal

基　　金：国家自然科学基金资助项目 ( 3 990 0 0 71)

摘　　要：目的　探讨血管紧张素Ⅰ转换酶 (ACE)基因插入 /缺失 (I/D)多态性与 2型糖尿病肾病发生和发展的关系。方法　对 2 98例 2型糖尿病患者 (T2DM总组 )和 87名非糖尿病正常人 (Non DM组 )进行病例对照研究。将T2DM总组分为无糖尿病肾病 (DN 0 )组和糖尿病肾病 (TDN)组 ,后者进一步分为微量蛋白尿 (DN 1)组和临床蛋白尿 (DN 2 )组。采用聚合酶链反应 4 %琼脂糖凝胶电泳法检测ACE基因内含子 16的 1个 2 87bp的Alu顺序I/D型多态标志。结果　① 2型糖尿病未合并肾病与合并肾病及肾功能不全等各亚组之间基因型频率的差异无显著性 (DN 0、DN 1和DN 2组DD∶DI∶II别为 2 7.5 %∶4 3.8%∶2 8.8%、2 3.3%∶39.5 %∶37.2 %和2 2 .5 %∶4 7.2 %∶30 .3% )。②基因型组合DD +DI与II各组间比较的差异无显著性。③等位基因频率的差异无显著性 (DN 0、DN 1和DN 2组D∶I分别为 4 9.4 %∶5 0 .6 %、4 3.0 %∶5 7.0 %和 4 6 .1%∶5 3.9% )。结论　ACEI/D多态性与Objective To investigate whether ACE gene I/D polymorphism is a predictor of onset and progression of diabetic nephropathy.Methods A case-control study for a total of 385 Chinese subjects including 298 patients with type 2 diabetes mellitus(T2DM) and 87 non-diabetes(Non-DM) for controls were performed. The T2DM group was subdivided into non- diabetic nephropathy(DN-0; n=80) and diabetic nephropathy(TDN; n=218) groups. The latter group was further subdivided into microalbuminuric nephropathy(DN-1; n=129)group and severe diabetic nephropathy(DN-2; n=89) group. The ACE gene insertion/deletion polymorphism was determind by PCR- 4% agarose gel electrophoresis.Results ①No significant difference in genotype distribution among the DN-0, DN-1 and DN-2 groups (DD∶DI∶II=27.5%∶43.8%∶28.8%/ 23.3%∶39.5%∶37.2%/ 22.5%∶47.2%∶30.3%, P>0.05) were observed. ②There was no significant difference between DD+DI and II (P>0.05). ③There was also no difference in allelic frequencies among all groups(P>0.05).Conclusion ACE gene I/D polymorphism is not associated with the development of Type 2 diabetic nephropathy among Chinese.

关 键 词：血管紧张素Ⅰ转换酶 基因多态性 2型糖尿病肾病 2型糖尿病 聚合酶链反应 琼脂糖凝胶电泳法 

分 类 号：R587.2[医药卫生—内分泌]